CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID

Bezeichnung:CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID

CAS-Nr99792-79-7

Englisch Name:CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID

CBNumberCB9497227

SummenformelC25H22Cl2O10S

Molgewicht585.41

MOL-Datei99792-79-7.mol

CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID physikalisch-chemischer Eigenschaften

Dichte	1.661
storage temp.	-20°C (-15°C to -25°C)
Löslichkeit	DMF: 30 mg/ml,DMSO: 30 mg/ml,PBS (pH 7.2): 10 mg/ml
Aggregatzustand	powder
Farbe	Brown to reddish brown

Sicherheit

WGK Germany	3
HS Code	29400090

CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung
Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate for β-galactosidase. Upon enzymatic cleavage by β-galactosidase, chlorophenol red is released, which can be quantified by colorimetric detection at 570 nm as a measure of β-galactosidase activity.
Chemische Eigenschaften
Crystal
Verwenden
Colorimetric determination of β-Gal activity in cell extracts and ELISA.
Verwenden
Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):
- in paper-based cell-free protein expression system for target RNA detection
- in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene
- in colorimetric assay for bacteria quantification
Verwenden
Chlorophenol Red-β-D-galactopyranoside measures β-galactosidase expression levels in transfected cells. Chlorophenol Red-β-D-galactopyranoside is a substrate of β-Galactosidase.
Allgemeine Beschreibung
Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.
Biochem/physiol Actions
Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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