7-(β-D-Glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-on

Bezeichnung:7-(β-D-Glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-on

CAS-Nr18997-57-4

Englisch Name:4-Methylumbelliferyl-beta-D-glucopyranoside

CBNumberCB8853266

SummenformelC16H18O8

Molgewicht338.31

MOL-Datei18997-57-4.mol

Synonyma

7-(β-D-Glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-on

7-(β-D-Glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-on physikalisch-chemischer Eigenschaften

Schmelzpunkt	258-263 °C (dec.)
Siedepunkt	394.46°C (rough estimate)
Dichte	1.522
Brechungsindex	1.4430 (estimate)
storage temp.	Sealed in dry,Store in freezer, under -20°C
Löslichkeit	DMF: 5 mg/ml; DMSO: 50 mg/ml
pka	12.73±0.70(Predicted)
Aggregatzustand	Powder
Farbe	White to off-white
Wasserlöslichkeit	Soluble in DMF or water
maximale Wellenlänge (λmax)	320nm(Buffer)(lit.)
BRN	94672
CAS Datenbank	18997-57-4(CAS DataBase Reference)

Sicherheit

Kennzeichnung gefährlicher	T+
R-Sätze:	26/27/28
S-Sätze:	22-24/25
WGK Germany	3
F	3-10-21
HS Code	29389090

Gefahreninformationscode (GHS)

4-Methylumbelliferyl-beta-D-glucopyranoside Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R26/27/28:Sehr giftig beim Einatmen, Verschlucken und Berührung mit der Haut.
S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
Beschreibung
4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease. Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.
Chemische Eigenschaften
White Powder
Verwenden
4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:
- in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
- in β-glucosidase assay during yeast fermentation
- to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)
Verwenden
A fluorogenic substrate in the assay of ?-glucosidase. Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15
Verwenden
A fluorogenic substrate in the assay of ?-glucosidase.Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15
Definition
ChEBI: A beta-D-glucoside having a 4-methylumbelliferyl substituent at the anomeric position.
Biochem/physiol Actions
4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci.
läuterung methode
4-Methylumbellifer-7-yl--Dglucopyranoside crystallises as the half hydrate from hot H2O. [Constantzas & Kocourek Collect Czech Chem Commun 24 1099 1959, De Re et al. Ann Chim (Rome) 4 9 2089 1959, Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 III/IV 5152, 8 IV 433, 18/7 V 616.]

4-Methylumbelliferyl-beta-D-glucopyranoside Upstream-Materialien And Downstream Produkte

7-(β-D-Glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-on Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

18997-57-4, 4-Methylumbelliferyl-beta-D-glucopyranoside Verwandte Suche:

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