PR-619

Bezeichnung:PR-619

CAS-Nr2645-32-1

Englisch Name:PR-619

CBNumberCB82656906

SummenformelC7H5N5S2

Molgewicht223.28

MOL-Datei2645-32-1.mol

PR-619 physikalisch-chemischer Eigenschaften

Schmelzpunkt	210℃
Siedepunkt	406.0±45.0 °C(Predicted)
Dichte	1.61±0.1 g/cm3(Predicted)
storage temp.	-20°C
Löslichkeit	DMSO: soluble5mg/mL (clear solution)
pka	-0.19±0.50(Predicted)
Aggregatzustand	powder
Farbe	white to beige
Stabilität	Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month.

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	22-37/38-41-43
S-Sätze:	26-36/37/39
WGK Germany	3

Gefahreninformationscode (GHS)

PR-619 Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung
PR-619 (2645-32-1) is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs; 5-20 μM)1. PR-619 is highly useful for preserving ubiquitinated proteins during cell lysis. DUBs become unregulated in cell lysates and will act quickly to deubiquitinate proteins of interest during cell lysis. To preserve maximum amounts of ubiquitinated protein, include PR-619 (50-100 μM) in lysis buffers. PR-619 is also useful for interrogating the role of DUBs in intact cell systems2, and treatment of cells results in an overall accumulation of ubiquitinated proteins and many other phenotypes.
Verwenden
PR-619 has been used:
- as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells
- as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction
- in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays
Verwenden
PR 619 is a broad-range DUB inhibitor. A selective and general inhibitior of cellular DUB activity without direct impairment of proteasomal proteolysis. Used in the preparation of thiazolopyridines an d bisthiazolopyridines.
Biochem/physiol Actions
PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity.
Lager
Store at -20°C
Einzelnachweise
1) Altun?et al. (2011),?Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes; Chem. Biol.,?18?1401 2) Seiberlich?et al. (2012),?The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: implications for neurodegenerative diseases; Biochim. Biophys. Acta,?1823?2057

PR-619 Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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