Carboxypeptidase B

Bezeichnung:Carboxypeptidase B

CAS-Nr9025-24-5

Englisch Name:CARBOXYPEPTIDASE B

CBNumberCB7435771

SummenformelC31H38N4O7S

Molgewicht610.72102

MOL-Datei9025-24-5.mol

Synonyma

Carboxypeptidase B

Carboxypeptidase B physikalisch-chemischer Eigenschaften

storage temp.	-20°C
Aggregatzustand	powder
Farbe	white

Sicherheit

Kennzeichnung gefährlicher	Xi,B,Xn
R-Sätze:	36-42-36/37/38
S-Sätze:	26-36-36/37-24-22
WGK Germany	3

Gefahreninformationscode (GHS)

CARBOXYPEPTIDASE B Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36:Reizt die Augen.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Verwenden
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.
Verwenden
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.
Allgemeine Beschreibung
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators.
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Carboxypeptidase B Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

9025-24-5, CARBOXYPEPTIDASE B Verwandte Suche:

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