FLUORESCEIN-12-DUTP

FLUORESCEIN-12-DUTP Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  Fluorescein-12-dUTP is used as a substrate for:
  
  • terminal transferase
  • DNA polymerase I (holoenzyme and Klenow fragment),
  • Taq DNA polymerase
  • reverse transcriptase (e.g., from AMV and M-MuLV).
  Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

FLUORESCEIN-12-DUTP Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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