Disuccinimidyl glutarate Struktur

Disuccinimidyl glutarate

Bezeichnung:Disuccinimidyl glutarate

CAS-Nr79642-50-5

Englisch Name:Disuccinimidyl glutarate

CBNumberCB0373852

SummenformelC13H14N2O8

Molgewicht326.26

MOL-Datei79642-50-5.mol

Disuccinimidyl glutarate physikalisch-chemischer Eigenschaften

Schmelzpunkt	168-172 °C
Siedepunkt	485.1±55.0 °C(Predicted)
Dichte	1.53±0.1 g/cm3(Predicted)
storage temp.	-20°C
Löslichkeit	Soluble in dimethylsulfoxide and dimethylformamide.
Aggregatzustand	Solid
Farbe	White to Off-White
Wasserlöslichkeit	Partially soluble in water.
Sensitive	Moisture Sensitive
BRN	8575145
Stabilität	Hygroscopic, Moisture Sensitive, Unstable in Aqueous Solution
InChI	InChI=1S/C13H14N2O8/c16-8-4-5-9(17)14(8)22-12(20)2-1-3-13(21)23-15-10(18)6-7-11(15)19/h1-7H2
InChIKey	LNQHREYHFRFJAU-UHFFFAOYSA-N
SMILES	C(ON1C(=O)CCC1=O)(=O)CCCC(ON1C(=O)CCC1=O)=O
CAS Datenbank	79642-50-5

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26-36
WGK Germany	3
F	10-21
HS Code	29280000

Gefahreninformationscode (GHS)

Disuccinimidyl glutarate Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Beschreibung
Disuccinimidyl glutarate (DSG) is a homobifunctional crosslinking agent that contains amine-reactive N-hydroxysuccinimide (NHS) ester groups at each end. DSG is membrane-permeable and non-cleavable. DSG can be combined with formaldehyde fixation to improve the detection of specific protein-DNA complexes.
Chemische Eigenschaften
DSG crosslinker (Disuccinimidyl glutarate; Di(N-succinimidyl) glutarate) is a homobifunctional crosslinking reagent that is membrane permeable. DSG protein crosslinker is often used in ChIP assays to determine DNA-protein binding interactions; using DSG and formaldehyde in a two-step crosslinking strategy greatly improves the ChIP assay. Disuccinimidyl crosslinkers include disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS), and bis-N-succinimidyl-(pentaethylene glycol) ester (BS(PEG)5)[1]. DSG crosslinking takes place through amine-reactive NHS esters at both ends of a 5-atom (7.7 angstrom) spacer arm. DSS (crosslinker arm length = 11.4Å), DSG (crosslinker arm length = 7.5Å), and disuccinimidyl tartrate (DST, crosslinker arm length = 5.8Å) are homobifunctional crosslinking reagents that react specifically with primary amines[2]. DSG crosslinker must be dissolved in an organic solvent, such as DMSO or DMF, and then added to an aqueous crosslinking reaction. The directly water-soluble version of Di(N-succinimidyl) glutarate (DSG) is BS2G.
Charakteristisch
Features of disuccinimidyl glutarate:
Reactive groups: NHS ester (both ends)
Reactive towards: amino groups (primary amines)
Non-cleavable
Water-insoluble (dissolve first in DMF or DMSO)
Membrane-permeable, allowing for intracellular crosslinking
Can increase crosslinking efficiency compared to DSS in some applications
Verwenden
Disuccinimidyl glutarate is used as a membrane-soluble, amine-reactive N-hydroxysuccinamide ester-based homobifunctional crosslinking reagent. It is a homobifunctionla cross-linking reagent, for ex. isolation of the insulin receptor.
Allgemeine Beschreibung
DSG crosslinker (Disuccinimidyl glutarate; Di(N-succinimidyl) glutarate) is a homobifunctional crosslinking reagent that is membrane permeable. DSG protein crosslinker is often used in ChIP assays to determine DNA-protein binding interactions; using DSG and formaldehyde in a two-step cross-linking strategy greatly improves the ChIP assay.
DSG crosslinking takes place through amine-reactive NHS esters at both ends of a 5-atom (7.7 angstrom) spacer arm. DSG crosslinker must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous crosslinking reaction. The directly water soluble version of Di(N-succinimidyl) glutarate (DSG) is BS2G.
Einzelnachweise
[1] JeeYoung Kim, Min Park, Gun Yong Sung. “Efficient Portable Urea Biosensor Based on Urease Immobilized Membrane for Monitoring of Physiological Fluids.” Biomedicines (2020).
[2] Petr Novak. “A top-down approach to protein structure studies using chemical cross-linking and Fourier transform mass spectrometry.” European Journal of Mass Spectrometry 9 6 (2003): 623–31.

Disuccinimidyl glutarate Upstream-Materialien And Downstream Produkte

Disuccinimidyl glutarate Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

79642-50-5, Disuccinimidyl glutarate Verwandte Suche:

Send Inquriy