MEM Chemische Eigenschaften,Einsatz,Produktion Methoden
R-Sätze Betriebsanweisung:
R34:Verursacht Verätzungen. R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren. S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen. S45:Bei Unfall oder Unwohlsein sofort Arzt zuziehen (wenn möglich, dieses Etikett vorzeigen). S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Verwenden
Adding supplements of amino acids to media stimulates growth and prolongs the viability of cells in culture. MEM Amino Acids (50x) solution concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro. It has been used in in vitro cultures, co-cultures and incubation media.
Verwenden
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.
Verwenden
Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.
Allgemeine Beschreibung
It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Biochem/physiol Actions
MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.
MEM Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
