地诺前列素氨丁三醇

生物活性靶点体外研究地诺前列素氨丁三醇试剂级价格

地诺前列素氨丁三醇

CAS号:38562-01-5
英文名:Dinoprost tromethamine
中文名:地诺前列素氨丁三醇
CBNumber:CB9394313
分子式:C24H45NO8
分子量:475.62
MOL File:38562-01-5.mol

地诺前列素氨丁三醇化学性质

熔点 :100-101°
储存条件 :-20°C
溶解度 :H₂O: 1 mg/mL Aqueous solutions are stable for 30 days at 2-8°C and for several months frozen at −0°C in single use aliquots. Avoid repeated freeze/thaw cycles.
形态 :powder
颜色 :white
Merck :13,7970
BRN :4087514
稳定性 :Hygroscopic
InChIKey :IYGXEHDCSOYNKY-RZHHZEQLSA-N

安全信息

危险品标志 :T
危险类别码 :60-22
安全说明 :53-45
WGK Germany :3
RTECS号 :UK8025000
F :8-10
海关编码 :2937500000

地诺前列素氨丁三醇性质、用途与生产工艺

生物活性 Dinoprost Tromethamine (Dinolytic, PGF2-alpha tham, Zinoprost, Prostin F2 alpha, Dinoprost Trometamol) 是一种合成的prostaglandin F2 alpha类似物，prostaglandin F2 alpha可通过直接作用于黄体素，刺激子宫肌层活性、使宫颈舒张、抑制黄体类固醇生成、诱导黄体溶解。
靶点

FP Receptor

Human Endogenous Metabolite

体外研究

Dinoprost tromethamine salt (Prostaglandin F2α tromethamine salt; 1 μM; for 24 hours) induces ER stress, autophagy, and apoptosis in goat luteal cells.
Dinoprost tromethamine salt (1 μM; for 24 hours) significantly increases the expression of GRP78 and UPR sensors.

Apoptosis Analysis

Cell Line:	Goat luteal cells
Concentration:	1 μM
Incubation Time:	For 24 hours
Result:	Significantly increased the apoptotic rate (15.62±3.12%).

Cell Autophagy Assay

Cell Line:	Goat luteal cells
Concentration:	1 μM
Incubation Time:	For 24 hours
Result:	There was extensive overlap between LC3 and LAMP1 in luteal cells and autophagolysosomes were formed in goat luteal cells.

Western Blot Analysis

Cell Line:	Goat luteal cells
Concentration:	1 μM
Incubation Time:	For 24 hours
Result:	The expression of GRP78 and UPR sensors including cleaved ATF6, phosphorylated-EIF2S1, EIF2S1, ATF4, phosphorylated-IRE1, autophagy-related protein LC3-II, and pro-apoptosis factor cleaved Caspase3 increased significantly in the cells.