灵芝酸D

生物活性靶点体外研究

灵芝酸D

CAS号:108340-60-9
英文名:Ganoderic acid D
中文名:灵芝酸D
CBNumber:CB92672936
分子式:C30H42O7
分子量:514.65
MOL File:108340-60-9.mol

灵芝酸D化学性质

沸点 :688.3±55.0 °C(Predicted)
密度 :1.22±0.1 g/cm3(Predicted)
储存条件 :Store at -20°C, protect from light
溶解度 :Soluble in methanol;
形态 :powder
酸度系数(pKa) :4.77±0.23(Predicted)
颜色 :White
水溶解性 :insoluble in water

灵芝酸D性质、用途与生产工艺

生物活性 Ganoderic acid D 是一种高度氧化的四环三萜类化合物 (tetracyclic triterpenoid)，是灵芝 (Ganoderma lucidum) 的主要活性成分。Ganoderic acid D 上调 SIRT3 的蛋白质表达并通过 SIRT3 诱导脱乙酰化的亲环蛋白 D (CypD)。Ganoderic acid D 抑制结肠癌细胞的能量重编程，包括结肠癌细胞中的葡萄糖摄取，乳酸产生，丙酮酸和乙酰辅酶的产生。Ganoderic acid D 诱导 HeLa 人宫颈癌细胞凋亡 (apoptosis)。
靶点

SIRT3

体外研究

Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC ₅₀ of 17.3 mM. Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells.
Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3.

Cell Viability Assay

Cell Line:	HeLa human cervical carcinoma cell line (CCL-2)
Concentration:	1, 5, 10, 20, 50 μM
Incubation Time:	24, 48, 72 hours
Result:	Reduced the cell survival rate in a dose- and time-dependent manner and had an IC ₅₀ value of 17.3 μM for 48 hours treatment.

Cell Cycle Analysis

Cell Line:	HeLa human cervical carcinoma cell line (CCL-2)
Concentration:	10, 50 μM
Incubation Time:	24, 48 hours
Result:	Induced G2/M phase arrest. Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM.

Apoptosis Analysis

Cell Line:	HeLa human cervical carcinoma cell line (CCL-2)
Concentration:	10, 50 μM
Incubation Time:	48 hours
Result:	Induced a morphological change typical of apoptosis in HeLa cells.