T67

生物活性靶点体外研究体内研究 T67 试剂级价格

T67

CAS号:195533-98-3
英文名:T67
中文名:T67
CBNumber:CB92496255
分子式:C13H6F6NNaO3S
分子量:393.2368292
MOL File:195533-98-3.mol

T67化学性质

储存条件 :Store at -20°C
溶解度 :DMSO: 125 mg/mL (317.87 mM)
形态 :A crystalline solid
颜色 :Off-white to light yellow

T67性质、用途与生产工艺

生物活性 Batabulin sodium (T138067 sodium) 是一种抗肿瘤剂，可与 β-微管蛋白同种型的子集共价且选择性地结合，从而破坏微管 (microtubule) 聚合。Batabulin sodium 影响细胞形态并导致细胞周期停滞，最终诱导凋亡性细胞死亡。
靶点

β-tubulin

体外研究

Batabulin (T138067; 30-300 nM; 24 hours; MCF7 cells) treatment shows approximately 25-30% tetraploid (4n) DNA content in cells, indicating an arrest at the G2/M cell-cycle boundary.
Batabulin (T138067; 30-300 nM; 24-48 hours; MCF7 cells) treatment shows 25-30% apoptosis. After a 48-hr exposure to 100 nM Batabulin, approximately 50-80% of the cell population is undergoing apoptosis.
Batabulin (T138067) binds covalently and selectively to a subset of the β-tubulin isotypes, thereby disrupting microtubule polymerization. Covalent modification occurs at a conserved Cys-239 shared by the β1, β2, and β4 tubulin isotypes. Cells exposed to Batabulin become altered in shape, indicating a collapse of the cytoskeleton, and show an increase in chromosomal ploidy.

Cell Cycle Analysis

Cell Line:	MCF7 cells
Concentration:	30 nM, 100 nM and 300 nM
Incubation Time:	24 hours
Result:	Showed an arrest at the G2/M cell-cycle boundary.

Apoptosis Analysis

Cell Line:	MCF7 cells
Concentration:	30 nM, 100 nM and 300 nM
Incubation Time:	24 hours or 48 hours
Result:	25-30% of cells showed the reduced DNA content characteristic of apoptotic cells.

体内研究

Batabulin (T138067; 40 mg/kg; intraperitoneal injection; once per week; on days 5, 12, and 19; male athymic nude mice) treatment impairs the growth of the drug-sensitive CCRF-CEM tumors.

Animal Model:	Male athymic nude mice ( nu/nu ) (6-8 week-old, 20-25 g) injected with CCRF-CEM cells
Dosage:	40 mg/kg
Administration:	Intraperitoneal injection; once per week; on days 5, 12, and 19
Result:	Impaired the growth of the drug-sensitive CCRF-CEM tumors.