DC_AC50

生物活性体外研究 DC_AC50 试剂级价格

DC_AC50

CAS号:497061-48-0
英文名:3-amino-N-(2-bromo-4,6-difluorophenyl)-6,7-dihydro-5H-cyclopenta[b]thieno[3,2-e]pyridine-2-carboxamide
中文名:DC_AC50
CBNumber:CB92001813
分子式:C17H12BrF2N3OS
分子量:424.26
MOL File:497061-48-0.mol

DC_AC50化学性质

沸点 :485.1±45.0 °C(Predicted)
密度 :1.759±0.06 g/cm3(Predicted)
储存条件 :2-8°C
溶解度 :Soluble in DMSO
酸度系数(pKa) :8.80±0.70(Predicted)
形态 :powder
颜色 :white to light brown

DC_AC50性质、用途与生产工艺

生物活性 DC_AC50 是 Atox1 和 CCS (铜伴侣蛋白) 的双抑制剂。抑制细胞内铜伴侣可作为减少/防止获得性化疗耐药的一种策略。

体外研究

DC_AC50 exhibits IC ₅₀ values of 9.88 μM, 12.57 μM, 5.96 μM and 6.68 μM in Canine Abrams, Canine D1, human HOS and human MG63) cells, respectively.
DC_AC50 (0-10 μM)-treated cells are significantly less mitotically active, as demonstrated by decreased expression of phospho-histone H3 and cell cycle analysis.
DC_AC50 (10 μM) potentiates carboplatin-induced apoptosis in OSA cells and decreasesclonogenic survival.
DC_AC50 induces cell cycle arrest at both the 3 and 10 μM doses and DC_AC50 induces increase S phase cells dose-independently.
DC_AC50 (3 μM) inhibits the migration and of canine and human OSA cells.
DC_AC50 (2.5-10 μM) is highly efficient at inhibiting cancer cell proliferation (human lung cancer H1299 cells, leukaemia cancer K562 cells, breast cancer MDA-MB-231 cells and head and neck cancer 212LN cells) in a dose-dependent manner. DC_AC50 fails to exhibit any notable inhibition of the cell proliferation of human normal epithelial lung BEAS-2B cells or breast MCF-10A cells as control cells.

Cell Viability Assay

Cell Line:	Canine OSA (Abrams, D1 and human OSA (HOS, MG63) cells.
Concentration:	0-10 μM.
Incubation Time:	72 h.
Result:	Dose-dependently decreased viability of OSA cells.

Apoptosis Analysis

Cell Line:	Abrams and HOS cells.
Concentration:	1, 3 and 10 μM (10 μM Carboplatin).
Incubation Time:	24 h.
Result:	Potentiated carboplatin-induced apoptosis.