2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐

生物活性靶点体外研究 2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐试剂级价格

CAS号:1049741-55-0
英文名:4-(2-HYDROXYETHYLAMINO)-2-(4-METHOXYPHENYLAMINO)-PYRIMIDINE HYDROCHLORIDE
中文名:2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐
CBNumber:CB8668084
分子式:C13H17ClN4O2
分子量:296.75
MOL File:Mol file

2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐化学性质

储存条件 :−20°C
溶解度 :H₂O: 10 mg/mL, soluble
形态 :solid
颜色 :White to off-white

安全信息

危险品标志 :Xi
危险类别码 :36/37/38
安全说明 :26-36
WGK Germany :3

2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐性质、用途与生产工艺

生物活性 Cardiogenol C hydrochloride 是一种有效的细胞渗透性嘧啶诱导剂，可促使 ESCs 分化为心肌细胞 (ESCs differentiation into cardiomyocytes) (EC50=100 nM)。Cardiogenol C hydrochloride 还可以在有限的可塑性范围内对已经沿袭的祖细胞类型产生心肌生成效应。Cardiogenol C hydrochloride 是一种有用的心肌生成试剂，在动物模型中可以用作细胞移植研究中提高心脏修复的工具。
靶点

EC50: 100 nM (differentiation of ESCs into cardiomyocytes)

体外研究

Cardiogenol C hydrochloride (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF, nppa ) in P19 cells when it compares to untreated control cells.Cardiogenol C hydrochloride (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells.Cardiogenol C hydrochloride (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells.Cardiogenol C hydrochloride (1 μM; 35 days) addition from day 0 significantly increases myocardial differentiation and results in a significantly increased percentage of CBs with beating cardiomyocytes. This small moleculepromotes the development of beating cardiomyocytes in cardiovascular progenitor cell-derived cardiac bodies.Cardiogenol C hydrochloride (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells.

RT-PCR

Cell Line:	C2C12 cells
Concentration:	0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM
Incubation Time:	7 days
Result:	Increased ANF and NKX2.5 mRNA level as a dose-dependent manner.

Western Blot Analysis

Cell Line:	C2C12 cells
Concentration:	0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM
Incubation Time:	7 days
Result:	Increased cardiac Nav1.5 sodium channel protein levels.

Cell Proliferation Assay

Cell Line:	C2C12 cells
Concentration:	0.01 μM; 0.1 μM; 1 μM
Incubation Time:	7 days
Result:	Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.