ANTI-BCMA 抗体

Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] (ab253242)

BCMA was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab253242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253242 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: U266B1 whole cell lysate 10 μg (Input).
Lane 2: ab253242 IP in U266B1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253242 in U266B1 whole cell lysate.

双抗体夹心法测定标本中BCMA水平。用纯化的BCMA抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入BCMA，再与HRP标记的BCMA抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的BCMA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中BCMA浓度。
Anti-BCMA Antibody是一类可以特异性结合BCMA的多克隆抗体，主要用于检测BCMA的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。