锌原卟啉

生物活性靶点体外研究体内研究锌原卟啉试剂级价格

锌原卟啉

CAS号:15442-64-5
英文名:PROTOPORPHYRINATO ZINC
中文名:锌原卟啉
CBNumber:CB8208833
分子式:C34H31N4O4Zn-
分子量:625.03
MOL File:15442-64-5.mol

锌原卟啉化学性质

RTECS号 :ZG9210300
储存条件 :Inert atmosphere,2-8°C
溶解度 :DMSO : 50 mg/mL (79.87 mM; Need ultrasonic)H2O : < 0.1 mg/mL (insoluble)
形态 :Crystalline
颜色 :Dark red to purple
水溶解性 :Insoluble in water. Soluble in ethanol, and DMSO, pyridine, or dimethyl formamide.
稳定性 :Hygroscopic
EPA化学物质信息 :Zincate(2-), [7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-.kappa.N21,.kappa.N22,.kappa.N23,.kappa.N24]-, dihydrogen, (SP-4-2)- (15442-64-5)

安全信息

WGK Germany :3
TSCA :Yes

锌原卟啉性质、用途与生产工艺

生物活性 Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) 是一种具有口服活性的，竞争性的血红素加氧酶-1 (HO-1) 抑制剂，显着减弱间苯三酚 (PG) 对 H2O2 的保护作用。Zinc Protoporphyrin 可用作个体孕妇和儿童缺铁的筛查标志物，也用于评估人群铁状态与血红蛋白浓度的组合。Zinc Protoporphyrin 具有抗癌活性。
靶点

Human Endogenous Metabolite

体外研究

Zinc Protoporphyrin (Zn(II)-protoporphyrin IX; 5 μM; 72 hours) causes the fraction of late apoptotic and necrotic cells increasing from 10.9% in controls to 30.4% after 72 h.
Zinc Protoporphyrin (1.25-40 μM; 48 or 72 hours) exerts cystostatic/cytotoxic effects against tumor cells.
Zinc Protoporphyrin (2.5, 5 μM; 48 or 72 hours) results in dose- and time-dependent reduction of cells in G1 phase of the cell cycle.
Zinc Protoporphyrin (1.25-40 μM; 48 hours) leads to accumulation of cleaved (active) caspase-3.

Apoptosis Analysis

Cell Line:	C-26 cells
Concentration:	5 μM
Incubation Time:	72 hours
Result:	The fraction of late apoptotic and necrotic cells increased from 10.9% in controls to 30.4% after 72 h.

Cell Cytotoxicity Assay

Cell Line:	C-26 and MDA-MB231 cells
Concentration:	1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time:	48 or 72 hours
Result:	Exerted cystostatic/cytotoxic effects against tumor cells.

Cell Cycle Analysis

Cell Line:	C-26 cells
Concentration:	2.5, 5 μM
Incubation Time:	48 or 72 hours
Result:	Resulted in dose- and time-dependent reduction of cells in G1 phase of the cell cycle.

Western Blot Analysis

Cell Line:	C-26 cells
Concentration:	1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time:	48 hours
Result:	Leaded to accumulation of cleaved (active) caspase-3.

体内研究

Zinc Protoporphyrin (12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.; from day 7 to 19) exerts dose-dependent antitumor effects manifested by the retardation of tumor growth.

Animal Model:	BALB/c mice inoculated with C-26 cells
Dosage:	12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.
Administration:	IP or PO; from day 7 to 19
Result:	Exerted dose-dependent antitumor effects manifested by the retardation of tumor growth.