野菊花内酯

生物活性体外研究体内研究野菊花内酯试剂级价格

野菊花内酯

CAS号:62687-22-3
英文名:handelin
中文名:野菊花内酯
CBNumber:CB81326400
分子式:C32H40O8
分子量:552.66
MOL File:62687-22-3.mol

野菊花内酯化学性质

沸点 :731.0±60.0 °C(Predicted)
密度 :1.34±0.1 g/cm3 (20 ºC 760 Torr)
形态 :Solid
酸度系数(pKa) :14.34±0.70(Predicted)
颜色 :White to off-white

野菊花内酯性质、用途与生产工艺

生物活性 Handelin 是来自Chrysanthemum boreale 的愈创木酚内酯二聚体，它通过下调 NF-κB 信号传导和促炎性细胞因子的产生而具有强大的抗炎活性。

体外研究

Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling.
Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin.

RT-PCR

Cell Line:	RAW 264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:	5 hours
Result:	iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed.

Western Blot Analysis

Cell Line:	RAW 264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:
Result:	Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.

体内研究

Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model .

Animal Model:	Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan
Dosage:	10 mg/kg, 20 mg/kg
Administration:	Oral administration; for 30 mintues
Result:	Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.