绿僵菌素B, 来源于绿僵菌属
- CAS号:2503-26-6
- 英文名:destruxin B
- 中文名:绿僵菌素B, 来源于绿僵菌属
- CBNumber:CB81304031
- 分子式:C30H51N5O7
- 分子量:593.761
- MOL File:2503-26-6.mol
- 熔点 :234 °C
- 沸点 :875.0±65.0 °C(Predicted)
- 密度 :1.17±0.1 g/cm3(Predicted)
- 储存条件 :-20°C
- 溶解度 :Soluble in DMSO (up to 10 mg/ml)
- 形态 :solid
- 酸度系数(pKa) :13.54±0.70(Predicted)
- 颜色 :White
- 稳定性 :Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
- 海关编码 :2933790090
绿僵菌素B, 来源于绿僵菌属性质、用途与生产工艺
- 生物活性 Destruxin B 是从昆虫病原真菌中分离的绿僵菌 (Metarhizium anisopliae)，是一种具有杀虫和抗癌活性的环肽。 Destruxin B 通过 Bcl-2 家族依赖的线粒体途径在人类非小细胞肺癌细胞中诱导细胞凋亡。Destruxin B 显著激活 caspase-3，并在体外和体内通过 caspase 介导的细胞凋亡减少肿瘤细胞增殖。
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靶点
Bcl-2
Caspase-3
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体外研究
Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC 50 s of 4.1 μM and 4.9 μM, respectively.
Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells.
Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners.
Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner .
Cell Proliferation Assay
Cell Line: H1299 cells Concentration: 1, 5, 10, 20, 30 μM Incubation Time: 48 hours Result: Inhibited H1299 cells proliferation of H1299 cells with an IC 50 of 4.1 μM. Apoptosis Analysis
Cell Line: Lung adenocarcinoma A549 cells Concentration: 1, 5, 10, 20, 30 μM Incubation Time: 48 hours Result: Induces caspase-dependent Lung adenocarcinoma A549 cells death. Cell Viability Assay
Cell Line: HT-29 human colorectal cancer cells Concentration: 1.25, 2.50, 5.00, 10.00 and 20.00 μM Incubation Time: 72 hours Result: The IC 50 s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively. Western Blot Analysis
Cell Line: A549 cells Concentration: 10 or 20 μM Incubation Time: 12 and 24 hours Result: Regulates Mcl-1 and PUMA. -
体内研究
Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners.
Animal Model: Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival Dosage: Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg) Administration: Injection; daily; for 6 weeks Result: Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.
- 更新日期：2024/11/08
- 产品编号：HY-N6690
- 产品名称：绿僵菌素B, 来源于绿僵菌属 Destruxin B
- CAS编号：2503-26-6
- 包装：1mg
- 价格：7800元
- 更新日期：2023/10/26
- 产品编号：HY-N6690
- 产品名称：绿僵菌素B, 来源于绿僵菌属 Destruxin B
- CAS编号：2503-26-6
- 包装：5mg
- 价格：27500元
- 公司名称：APOLLO-NMR
- 联系电话：--
- 电子邮件：sales@apolloscientific.co.uk
- 国家：英国
- 产品数：6084
- 优势度：88
