未消化麦醇溶蛋白肽GLIADIN P31-43
- CAS号:176326-01-5
- 英文名:L-Tyrosine, L-leucylglycyl-L-glutaminyl-L-glutaminyl-L-glutaminyl-L-prolyl-L-phenylalanyl-L-prolyl-L-prolyl-L-glutaminyl-L-glutaminyl-L-prolyl-
- 中文名:未消化麦醇溶蛋白肽GLIADIN P31-43
- CBNumber:CB78590546
- 分子式:C71H102N18O20
- 分子量:1527.68
- MOL File:176326-01-5.mol
- 沸点 :1968.7±65.0 °C(Predicted)
- 密度 :1.371±0.06 g/cm3(Predicted)
- 形态 :Solid
- 酸度系数(pKa) :3.11±0.10(Predicted)
- 颜色 :White to off-white
未消化麦醇溶蛋白肽GLIADIN P31-43性质、用途与生产工艺
- 生物活性 Gliadin p31-43 是一种未消化的麦醇溶蛋白肽,可诱导肠道内的先天性免疫反应并干扰内吞运输。Gliadin p31-43 可用于乳糜泻的研究。
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体外研究
Gliadin p31-43 (100 μg/mL; 30 minutes-6 hours) treatment induces the MyD88/TLR7 complexes, and activates downstream signalling by activating MAPKs, ERK, JNK and p38). Gliadin p31-43 increases the levels of the phosphorylated forms of pY-ERK, JNK (pY-JNK) and p38 (pY-p38).
Gliadin p31-43 treatment increases NF-κB phosphorylation in CaCo-2 cells from 0.45 in control cells to 0.86. Gliadin p31-43 treatment induces a significant increase in levels of the MxA protein. The levels of the IFN-α 7 and 17 mRNAs are also analysed after Gliadin p31-43 treatment.
In CaCo-2 cells, Gliadin p31-43 localizes to the early endosomes and delays vesicular trafficking. Gliadin p31-43 interferes with the correct localization of the growth factor regulated tyrosine kinase substrate (HRS) to early endosomes, delaying the maturation of the endocytic vesicles.
Western Blot Analysis
Cell Line: CaCo-2 cells Concentration: 100 μg/mL Incubation Time: 30 minutes, 3 hours, 6 hours Result: Showed the increase in formation of the MyD88/TLR7 complex, and increased in the level of TLR7. -
体内研究
Gliadin p31-43 (10 μg; intraluminally injection) shows a sequence-specific spontaneous ability to form structured oligomers and aggregates in vitro and induced activation of the apoptosis-associated speck-like (ASC) complex.
The increment of IL-1β indicates the activation of the inflammasome caspase-1 pathway in the small intestine mucosa by oral administration of Gliadin p31-43 (20 μg) in wild type C57Bl/6 mice. Gliadin p31-43 has an intrinsic propensity to form oligomers which trigger the NLRP3 inflammasome.
- 更新日期:2024/08/19
- 产品编号:HY-P3151
- 产品名称:Gliadin p31-43
- CAS编号:
- 包装:1 mg
- 价格:500元
- 更新日期:2024/08/19
- 产品编号:HY-P3151
- 产品名称:Gliadin p31-43
- CAS编号:176326-01-5
- 包装:5 mg
- 价格:1250元
- 公司名称:MedChemExpress
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- 电子邮件:sales@medchemexpress.com
- 国家:美国
- 产品数:6398
- 优势度:58