MONOCLONAL ANTI-GAPDH 小鼠抗

小鼠单克隆抗体to GAPDH

Mouse

适用于: WB, IHC-P, Flow Cytmore details

与反应: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Chinese hamster

预测可用于: Cynomolgus monkey

Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1micro;g/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : NIH 3T3 Whole Cell Lysate
Lane 3 : PC12 Whole Cell Lysate
Lysates/proteins at 20micro;g per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - HL - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab9484 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab13340

GAPDH或G3PDH是甘油醛-3-磷酸脱氢酶（glyceraldehyde-3-phosphate dehydrogenase）的英文缩写。GAPDH是糖酵解反应中的一个酶，广泛分布于各种组织中的细胞。 GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30-40kDa的亚基组成，分子量146kDa，检测条带大约在36kDa。
GAPDH基因几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参。因为GAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，因此在使用GAPDH内参抗体时，将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量，然后才进行样品与样品之间的比较。