红陪酚

生物活性靶点体外研究体内研究红陪酚试剂级价格

红陪酚

CAS号:569-77-7
英文名:PURPUROGALLIN
中文名:红陪酚
CBNumber:CB7329636
分子式:C11H8O5
分子量:220.18
MOL File:569-77-7.mol

红陪酚化学性质

熔点 :275 °C (dec.)(lit.)
沸点 :321.11°C (rough estimate)
密度 :1.3824 (rough estimate)
折射率 :1.5140 (estimate)
储存条件 :−20°C
溶解度 :DMSO (Slightly), Methanol (Slightly)
形态 :Solid
酸度系数(pKa) :6.75±0.20(Predicted)
颜色 :Very Dark Brown
Merck :7946
稳定性 :Air Sensitive
LogP :2.050 (est)

安全信息

危险品标志 :Xi
危险类别码 :36/37/38
安全说明 :26-36
WGK Germany :3
RTECS号 :DE8380000

红陪酚性质、用途与生产工艺

生物活性 Purpurogallin 是从栎属植物中提取的天然酚，具有强的黄嘌呤氧化酶 (Xanthine Oxidase) 抑制活性，其 IC50 为 0.2 µM。Purpurogallin 具有抗氧化和抗炎作用。
靶点

IC50: 0.2 µM (xanthine oxidase)

体外研究

Purpurogallin (50 or 100 µM; 7 or 25 hours; BV2 murine microglial cells) treatment attenuates the production of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) by suppressing their mRNA and protein expression in LPS-stimulated BV2 microglial cells.
Purpurogallin (100 µM; 75-120 minutes; BV2 murine microglial cells) exhibits anti-inflammatory properties by suppressing the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase signaling pathways in LPS-stimulated BV2 microglial cells.

RT-PCR

Cell Line:	BV2 murine microglial cells
Concentration:	50 or 100 µM
Incubation Time:	7 or 25 hours
Result:	Attenuated the production of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) by suppressing their mRNA and protein expression.

Western Blot Analysis

Cell Line:	BV2 murine microglial cells
Concentration:	100 µM
Incubation Time:	75 minutes, 90 minutes, 120 minutes
Result:	Suppressed the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase signaling pathways.

体内研究

Purpurogallin (100-400 μg/kg; intraperitoneal injection; for 48 or 72 hours; male Sprague-Dawley rats) exerts its neuroinflammation effect through the dual effect of inhibiting IL-6 and TNF-α mRNA expression and reducing HMGB1 protein and mRNA expression.

Animal Model:	Fifty-four male Sprague-Dawley rats (250-350 g) with subarachnoid hemorrhage (SAH)
Dosage:	100 μg/kg, 200 μg/kg, 400 μg/kg
Administration:	Intraperitoneal injection; for 48 or 72 hours
Result:	Dose-dependently reduced HMGB1 protein expression. High dose reduced TNF-α and HMGB1 mRNA levels.