Anti-c-Myc tag抗体(HRP)

Western blot - Anti-Myc tag antibody (ab9106)
Pais et al PLoS One. 2013;8(2):e56292. doi: 10.1371/journal.pone.0056292. Epub 2013 Feb 19. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Y. enterocolitica ΔHOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1 (as indicated) were grown in non-secreting conditions. Chloramphenicol was added (time?=?0 min) to stop bacterial protein synthesis and samples were taken at the indicated time points. Samples were analyzed by immunoblotting using ab9106, anti-HA, and anti-TEM antibodies.

双抗体夹心法测定标本中c-Myc tag水平。用纯化的c-Myc tag抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入c-Myc tag，再与HRP标记的c-Myc tag抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的c-Myc tag呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中c-Myc tag浓度。
Anti-c-Myc tag抗体(HRP)是一类可以特异性结合c-Myc tag的多克隆抗体，主要用于检测c-Myc tag的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。