ZZW-115 hydrochloride

生物活性体外研究体内研究 ZZW-115 hydrochloride 试剂级价格

CAS号:10122-45-9
英文名:ZZW-115 hydrochloride
中文名:ZZW-115 hydrochloride
CBNumber:CB69041617
分子式:
分子量:0
MOL File:Mol file

ZZW-115 hydrochloride化学性质

储存条件 :4°C, stored under nitroge
溶解度 :Ethanol: soluble
形态 :A solid
颜色 :Light brown to brown
水溶解性 :Water: 100 mg/mL (174.23 mM)

ZZW-115 hydrochloride性质、用途与生产工艺

生物活性 ZZW-115 hydrochloride 是一种有效的 NUPR1 抑制剂，Kd 为 2.1 μM，通过坏死和凋亡 (apoptosis) 诱导肿瘤细胞死亡。具有抗癌活性。

体外研究

ZZW-115 hydrochloride (0.1-33 μM; 72 hours) is efficient in killing cancer cells, with an IC ₅₀ in the range of 0.84 μM (ANOR) to 4.93 μM (HN14).
ZZW-115 hydrochloride (0-100 μM; 24-72 hours) is efficient to kill these tumor cells with an IC ₅₀ in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells).
ZZW-115 hydrochloride induces pancreatic cell death by necrosis and apoptosis. ZZW-115 hydrochloride treatment induces a decrease in ATP production and induces a ROS overproduction.
LDH release is significantly higher in ZZW-115 hydrochloride-treated cells (MiaPaCa-2, 02-063, LIPC, Foie8b, and HN14 cells) than in control cells in a concentration-dependent manner. Similarly, caspase 3/7 activity is also greater in ZZW-115 hydrochloride-treated cells. These experiments demonstrated that ZZW-115 hydrochloride exerted both pronecrotic and proapoptotic effects.

Cell Viability Assay

Cell Line:	ANOR cells, MiaPaCa-2, 02-063, 01008, LIPC, 02136, HN01,01046, AOIPC, Foie8b, HN14 cells
Concentration:	0.1- 33 μM
Incubation Time:	72 hours
Result:	Was efficient in killing cancer cells, with an IC ₅₀ in the range of 0.84 μM (ANOR) to 4.93 μM (HN14).

Cell Proliferation Assay

Cell Line:	U87, A375, U2OS, SaOS-2, HT29, SK-CO-1, LS174T, H1299 and H358, HepG2, PC3, THP-1, Daudi, Jurkat and MDA-MB-231 cells
Concentration:	0-100 μM
Incubation Time:	24 or 72 hours
Result:	Was efficient to kill these tumor cells with an IC ₅₀ in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells).

体内研究

ZZW-115 hydrochloride (0.5-5 mg/kg; injection; daily for 30 days) inhibits the growth of pancreatic xenografted tumors.
ZZW-115 hydrochloride (5 mg/kg for 30 days; immunocompetent C57BL/6 mice were orthotopically implanted with Panc02 cells) treatment shows the tumor size is almost unmeasurable in some cases.

Animal Model:	NMRI-Foxn1nu/Foxn1nu mice (nude mice) xenografted with MiaPaCa-2 cells
Dosage:	5, 2.5, 1.0, or 0.5 mg/kg
Administration:	Injection; daily for 30 days
Result:	When the mice were injected with 5 mg/kg ZZW-115 hydrochloride, the tumors stopped growing a few days after treatment and their size decreased progressively, almost disappearing at the end of the treatment.