兔抗PKC ALPHA多克隆抗体

兔多克隆抗体to PKC alpha

Rabbit

适用于: WB

与反应: Mouse, Rat, Cow, Human

Synthetic peptide: SYVNPQFVHPILQSAV , corresponding to amino acids 657-672 of Rat PKC alpha.

Western blot - Anti-PKC alpha antibody (ab4124)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab4124 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab4124 was shown to recognize PKC alpha in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab4124 and ab8245 (loading control to GAPDH) were diluted to 1/250 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

Rabbit anti-PKC alpha Polyclonal Antibody是以anti-PKC alpha为抗原以兔为宿主制得的免疫抗体，可以特异性结合PKC alpha。主要用于检测PKC alpha的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。