Suc-Leu-Leu-Val-Tyr-AMC

Suc-Leu-Leu-Val-Tyr-AMC化学性质

沸点 :1116.8±65.0 °C(Predicted)
密度 :1.249±0.06 g/cm3(Predicted)
储存条件 :-20°C
溶解度 :Soluble in DMSO (up to 20 mg/ml), or in DMF (up to 10 mg/ml)
形态 :Lyophilized solid
酸度系数(pKa) :4.69±0.10(Predicted)
颜色 :White to Off-White
序列 :Suc-Leu-Leu-Val-Tyr-AMC
稳定性 :Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20°C for up to 1 month.

安全信息

Suc-Leu-Leu-Val-Tyr-AMC性质、用途与生产工艺

生物活性 Suc-Leu-Leu-Val-Tyr-AMC 是一种荧光底物。
体外研究

Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety. The effect of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively.
多肽 Suc-Leu-Leu-Val-Tyr-AMC是一种具有荧光显色反应的底物多肽，C端是具有荧光型的香豆素，N端由丁二酸酐封闭，活性基团为羧基。生产Suc-Leu-Leu-Val-Tyr-AMC时，首先合成的是多肽片段Suc-Leu-Leu-Val，最后连接香豆素。
参考质量标准外观:白色粉末
纯度(HPLC) ≥98.0%
醋酸根含量≤12.0%
水分含量≤8.0%
肽含量≥80.0%
内毒素≤50EU/mg
氨基酸组成分析≤±10%

Suc-Leu-Leu-Val-Tyr-AMC上下游产品信息

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