ANTI-CD28抗体

小鼠单克隆抗体to CD28

与反应: Human

Unconjugated

适用于: Flow Cyt, IHC-Fr

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD28 (rh CD28; Catalog#11524-H08H; P10747-1; Met 1-Pro 152). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Immunocytochemistry/ Immunofluorescence - Anti-CD28 antibody [CB28] (ab193350)

Immunofluorescent staining of Jurkat cells using ab193350 followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to label nuclei (red).

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.