4-(1-(2-氨基-5-氯嘧啶-4-基)-2-(噻唑-2-基)-3-丁炔-2-醇

生物活性靶点体外研究体内研究 4-(1-(2-氨基-5-氯嘧啶-4-基)-2-(噻唑-2-基)-3-丁炔-2-醇试剂级价格

CAS号:1202764-53-1
英文名:4-(1-(2-amino-5-chloropyrimidin-4-yl)-2-(thiaol-2-yl)but-30-yn-2-ol
中文名:4-(1-(2-氨基-5-氯嘧啶-4-基)-2-(噻唑-2-基)-3-丁炔-2-醇
CBNumber:CB55582861
分子式:C19H16ClN5OS
分子量:397.88
MOL File:1202764-53-1.mol

4-(1-(2-氨基-5-氯嘧啶-4-基)-2-(噻唑-2-基)-3-丁炔-2-醇化学性质

沸点 :644.4±65.0 °C(Predicted)
密度 :1.53±0.1 g/cm3(Predicted)
储存条件 :Store at -20°C
溶解度 :DMSO: 250 mg/mL (628.33 mM)
形态 :Solid
酸度系数(pKa) :10.71±0.29(Predicted)
颜色 :Light yellow to yellow

4-(1-(2-氨基-5-氯嘧啶-4-基)-2-(噻唑-2-基)-3-丁炔-2-醇性质、用途与生产工艺

生物活性 B022 是一种有效的选择性的 NF-κB 诱导激酶 (NIK) 抑制剂，Ki 为 4.2 nM。B022 可保护肝脏免受毒素引起的炎症，氧化应激和伤害。
靶点

Ki: 4.2 nM (NF-κB-inducing kinase (NIK))

体外研究

B022 (0-5 μM; 12 hours; Hepa1 cells) treatment suppresses NIK-induced p52 formation in a dose-dependent manner.
B022 (0-5 μM; 12 hours; Hepa1 cells) treatment for 8 h completely blocks NIK-induced expression of TNF-a, IL-6, iNOS, CCL2, and CXCL5.

Western Blot Analysis

Cell Line:	Hepa1 cells
Concentration:	0 μM, 0.5 μM, 5 μM
Incubation Time:	12 hours
Result:	Suppressed NIK-induced p52 formation in a dose-dependent manner.

RT-PCR

Cell Line:	Hepa1 cells
Concentration:	0 μM, 0.5 μM, 5 μM
Incubation Time:	12 hours
Result:	Dose-dependently blocked NIK-induced expression of chemokines, cytokines, and iNOS in these cells. Completely blocked NIK-induced expression of TNF-a, IL-6, iNOS, CCL2, and CXCL5.

体内研究

B022 (30 mg/kg; intravenous injection; twice a day; for 10 days; STOP-NIK male mice) treatment inhibits NIK-triggered liver inflammation and injury in STOP-NIK mice infected with cre adenoviruses.

Animal Model:	STOP-NIK male mice (8 weeks) infected with Ad-cre
Dosage:	30 mg/kg
Administration:	Intravenous injection; twice a day; for 10 days
Result:	Completely prevents the lethal effect of abnormally high levels of hepatic NIK in mice. Inhibited the majority of the deteriorating effects of aberrant activation of hepatic NIK.