EF-5
- CAS号:152721-37-4
- 英文名:EF-5
- 中文名:EF-5
- CBNumber:CB53339650
- 分子式:C8H7F5N4O3
- 分子量:302.16
- MOL File:152721-37-4.mol
- 密度 :1.66±0.1 g/cm3(Predicted)
- 储存条件 :Store at -20°C
- 溶解度 :DMSO : 125 mg/mL (413.69 mM)
- 形态 :Solid
- 酸度系数(pKa) :12.32±0.46(Predicted)
- 颜色 :White to off-white
EF-5性质、用途与生产工艺
- 生物活性 EF-5 (EF5; 2-Nitroimidazole) 是用于鉴定细胞缺氧的缺氧标记试剂。
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体外研究
Overexpression of CYPOR induces similar 2- to 4-fold increases in EF-5 binding and metabolic reduction of tirapazamine and CEN-209 in SiHa and HCT116 cell lines, and similar enhancement of γH2AX formation. EF-5 binding and metabolic reduction of the prodrugs are highly correlated in a panel of 14 hypoxic tumor cell lines.
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体内研究
EF-5 binding is a promising stratification biomarker for benzotriazine-N-oxide bioreductive prodrugs. In HCT116 xenografts, CYPOR overexpression also significantly increases EF-5 binding and CEN-209 reduction, and modification of tumor hypoxia causes similar changes to the bioreductive activation of both agents, resulting in a strong correlation between EF-5 binding and CEN209-induced DNA damage at the individual tumor level. Following intravenous injection of EF-5, binding and detection using a monoclonal antibody in 9L gliomas is specific and oxygen dependent. Detection of binding using fluorescence microscopy can be performed on frozen tissues; tissue sections can be counterstained with haematoxylin and eosin for light microscopic analysis. Alternatively, the distribution of hypoxia in a tumor can be inferred by examining individual tumor cells using flow cytometric techniques.
- 更新日期:2025/02/08
- 产品编号:HY-U00118
- 产品名称:EF-5 EF-5
- CAS编号:152721-37-4
- 包装:5mg
- 价格:900元
- 更新日期:2025/02/08
- 产品编号:HY-U00118
- 产品名称:EF-5 EF-5
- CAS编号:152721-37-4
- 包装:10mM * 1mLin DMSO
- 价格:990元
- 公司名称:MedChemexpress LLC
- 联系电话:021-58955995
- 电子邮件:sales@medchemexpress.cn
- 国家:美国
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- 公司名称:TargetMol Chemicals Inc.
- 联系电话:+1-781-999-5354 +1-00000000000
- 电子邮件:marketing@targetmol.com
- 国家:美国
- 产品数:32161
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- 公司名称:MedChemExpress
- 联系电话:--
- 电子邮件:sales@medchemexpress.com
- 国家:美国
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