ML334

生物活性靶点体外研究 ML334 试剂级价格

ML334

CAS号:1432500-66-7
英文名:ML334
中文名:ML334
CBNumber:CB53159380
分子式:C26H26N2O5
分子量:446.49
MOL File:1432500-66-7.mol

ML334化学性质

沸点 :693.1±55.0 °C(Predicted)
密度 :1.354±0.06 g/cm3(Predicted)
储存条件 :Store at 4°C, stored under nitrogen
溶解度 :DMSO: 40 mg/ml
形态 :A solid
酸度系数(pKa) :4.69±0.44(Predicted)
颜色 :White to off-white

ML334性质、用途与生产工艺

生物活性 ML334 (LH601A) 是一种有效的，细胞可渗透的 NRF2 激活剂，通过抑制 Keap1-NRF2 蛋白质相互作用来发挥作用。ML334 以 Kd 值为 1μM 来结合 Keap1 Kelch 域。ML334 刺激 NRF2 表达和核易位并诱导抗氧化反应元件 (ARE) 活性。
靶点

Ki: 1 μM (Keap1)

体外研究

ML334 (LH601A; 50-100 µM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h.
ML334 (LH601A; 50-100 µM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells.
ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells.

RT-PCR

Cell Line:	HEK293 cells
Concentration:	50 μM or 100 µM
Incubation Time:	6 hours, 16 hours
Result:	Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h.