灵菌红素

生物活性体外研究体内研究灵菌红素试剂级价格

灵菌红素

CAS号:82-89-3
英文名:PRODIGIOSIN
中文名:灵菌红素
CBNumber:CB5156304
分子式:C20H25N3O
分子量:323.43
MOL File:82-89-3.mol

灵菌红素化学性质

熔点 :151-152°
沸点 :554.3±50.0 °C(Predicted)
密度 :1.12±0.1 g/cm3(Predicted)
储存条件 :-20C
溶解度 :Acetonitrile: Soluble; Ethanol: Soluble; Methanol: Soluble
形态 :Dark red solid
酸度系数(pKa) :17.09±0.50(Predicted)
颜色 :Dark red

灵菌红素性质、用途与生产工艺

生物活性 Prodigiosin (Prodigiosine)，一种天然的红色素，是一种具有生物活性的次生代谢产物。Prodigiosin 是 Wnt/β-catenin 途径的有效抑制剂。Prodigiosin 具有抗菌，抗真菌，抗原生动物，抗疟疾，免疫抑制和抗癌特性。

体外研究

Prodigiosin (25-500 nM; 24 hours) treatment reduces the viability of breast cancer cells, with IC ₅₀ values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells.
Prodigiosin (25-500 nM; 24 hours) treatment significantly reduces the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin. Prodigiosin noticeably inhibits the phosphorylation of GSK3β at Ser9 in HEK293T cells, which is indicative of an increase in GSK3β activity.
Prodigiosin can inhibit proliferation and induce apoptosis in breast cancer cells.
Prodigiosin (25-500 nM; 24 hours) treatment dose-dependently blocks Wnt signaling activated by Wnt1, Wnt3, Wnt1/LRP6, Wnt3/LRP6, and Dishevelled 2 (DVL2) in transfected HEK293T cells. Prodigiosin treatment inhibits Wnt3A-CM-induced transcription in a dose-dependent manner. Prodigiosin inhibits transcription of the SuperTopFlash reporter activated by either Wnt transfection or Wnt3A treatment.
When applied to cultures of chytrid fungi Batrachochytrium dendrobatidis and B. salamandrivorans , Prodigiosin causes significant growth inhibition, with MIC values of 10 μM and 50 μM, respectively.

Cell Proliferation Assay

Cell Line:	MDA-MB-231 and MDA-MB-468 cells
Concentration:	10 nM, 25 nM, 50 nM, 100 nM, 250 nM, 500 nM, 1000 nM, 2500 nM, 5000 nM
Incubation Time:	24 hours, 48 hours
Result:	Reduced the viability of breast cancer cells, with IC ₅₀ values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells.

Western Blot Analysis

Cell Line:	HEK293T cells
Concentration:	50 nM, 100 nM, 250 nM, 500 nM
Incubation Time:	24 hours
Result:	Significantly reduced the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin.

体内研究

Prodigiosin (5 mg/kg; intraperitoneal injection; twice weekly; for 3 weeks) treatment significantly inhibits tumor growth. Prodigiosin treatment decreases tumor cell density and expression of the proliferation marker Ki-67.

Animal Model:	Female BALB/c nude mice injected with MDA-MB-231 cells
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; twice weekly; for 3 weeks
Result:	Significantly inhibited tumor growth in mice.