比色皿

中文名:比色皿
英文名:cuvette
规格:5mm，10mm，20mm，30mm，40mm，50mm，100mm
分类:玻璃、石英、石英玻璃
特性: 耐高温,耐腐蚀,耐有机酸碱溶液
应用:光学分析测定

比色皿相关产品

比色皿产品介绍

比色皿( 又名吸收池，样品池) 用来装参比液、样品液。比色皿是分光光度计的重要配件，常用比色皿的形状有方形、矩形和圆筒形, 容量一般为几毫升。也有用于少量试样的微型或超微型毛细管皿。

比色皿的制造工艺有两种, 一种是粘合剂粘合而成, 另一种是高温熔融而成。比色皿的材料通常来源于石英、熔凝硅石和光学玻璃。常用比色皿的形状有方形、矩形和圆筒形, 容量一般为几毫升。也有用于少量试样的微型或超微型毛细管皿。另外还有高、低温恒温比色皿。比色皿按照使用的波长范围分为可见光系列（称玻璃比色皿），紫外可见光系列（称石英比色皿），红外光系列（称红外石英比色皿）。

分类

比色皿按材质分为石英比色皿和玻璃比色皿两种。

紫外区的定义为190-400nm，石英适用波长190-2500nm，可用于紫外、可见区及近红外区；光学玻璃适用于波长320-2500nm，不适用于紫外区。

玻璃比色皿口沿处有“G”（glass玻璃），而石英比色皿口沿处有“Q”（Quartz石英）或者“S”（Silicide石英玻璃）。

清洗方法

1.比色皿用完后应当先用适当溶剂冲洗,注意里外都要洗到.如果溶剂无毒的话,可以装入一半溶剂后,用手指按住比色皿的两端,用力摇晃,次数应当是不少于三次。

2.然后用大量的自来水冲洗,这一步对水溶液和盐溶液非常重要。当然如果所用溶剂不亲水这步可以放弃。

3.最后再用去离子水清洗,注意里外都要洗到。如果溶剂不亲水的这一步也可放弃。

4.洗完后不要刻意的将比色皿擦干,虚放在比色皿盒内,不用盖上让其自然挥干。

注：

清洗最好在用后立即进行,否则样品干后就更难处理；

洗好的比色皿应当是透明,没有水迹的；

经常使用的比色皿可以在洗净后浸泡在纯水或分析纯乙醇里中保存。

管理维护

(1) 按照实验中所使用的波长来选择相应的比色皿( 玻璃或者石英) ，紫外光区用石英比色皿，而可见光区既可以使用玻璃比色皿，又可以使用石英比色皿。考虑到价格问题，可见光区选用玻璃比色皿。尽量做到专人专用或者专组专用，用完清理后就交回。这样不易搞混不同的比色皿，也不影响比色皿间的配对。

(2) 尽量做到每个实验每台紫外分光光度计有专用的配套比色皿，不相互混用。如有交叉使用，可记录在册，下次恢复正常。

(3) 用完即清洗，清洗后在通风阴凉处干燥，等彻底干燥后放入相应装具中。放置时，装具保持清洁干燥，比色皿应秉承“光面朝上，毛面在两侧”的原则，这样便于抓取两毛面拿出使用，不易弄污光面。

使用注意事项

(1) 拿取比色皿时，只能用手指接触两侧的毛玻璃，避免接触光学面。同时注意轻拿轻放，防止破损。

(2) 比色皿中不应长期盛放含有腐蚀玻璃物质的溶液。

(3) 比色皿高温后易爆裂，因此不应放在火焰或电炉上加热或干燥箱内烘烤。

(4) 当比色皿里面被污染，应用无水乙醇清洗，并晾干或及时擦拭干净。

(5) 比色皿的透光面不应与硬物或脏物接触。

（6）盛装溶液时，高度应为比色皿的2 /3 处，光学面如有残液可先用滤纸轻轻吸附，然后再用镜头纸或丝绸擦拭。

（7）比色皿中的液体，应沿毛面倾斜，慢慢倒掉，不要将比色皿翻转，直接口向下放在干净的滤纸上吸干剩余液，然后用蒸馏水冲洗比色皿内部倒掉(操作同上)避免液体外流，使第2次测量时不用擦拭比色皿，不致因擦拭带来的误差。

（8）比色前将各个比色皿中装入蒸馏水，在比色波长下进行比较，误差在±0.001吸光度以内的比色皿选出4-8个进行比色测定，可避免因比色皿差异造成测量误差。

（9）比色皿在使用后,应立即用水冲洗干净。必要时可用1:1的盐酸浸泡,然后用水冲洗干净。

前人用过的经验：

1 使用前将比色皿在2%的硝酸溶液中浸泡24小时，然后用水，蒸馏水依次冲洗干净，擦干。

2 比色前将各个比色皿中装入蒸馏水，在比色波长下进行比较，误差在±0.001吸光度以内的比色皿选出4-8个进行比色测定，可避免因比色皿差异造成测量误差。

3 比色皿中的液体，应沿毛面倾斜，慢慢倒掉，不要将比色皿翻转，直接口向下放在干净的滤纸上吸干剩余液，然后用蒸馏水冲洗比色皿内部倒掉(操作同上)避免液体外流，使第2次测量时不用擦拭比色皿，不致因擦拭带来的误差。

比色皿供应商筛选

Sigma-Aldrich西格玛奥德里奇(上海)贸易有限公司

联系电话： 021-61415566 800-8193336

电子邮件： orderCN@merckgroup.com

国家：中国

产品数： 51456

优势度： 80

北京普天同创生物科技有限公司

联系电话： 18153089275

电子邮件： 3032079623@qq.com

国家：中国

产品数： 22695

优势度： 58

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