辣根过氧化物酶标记 肿瘤坏死因子抗体

 兔单克隆抗体[EPR1653] to TNF Receptor II (HRP)

Rabbit

HRP

适用于: IHC-P, WB

与反应: Human

Synthetic peptide within Human TNF Receptor II aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody(HRP) (ab200006)

IHC image of TNF Receptor II staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab200006 at 1/50 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

辣根过氧化物酶标记肿瘤坏死因子抗体是一类以肿瘤坏死因子为抗原的多克隆抗体，可以特异性结合肿瘤坏死因子，主要用于检测肿瘤坏死因子的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。