4,4'-(dithiodicarbonothioyl)dimorpholine

生物活性靶点体外研究体内研究 4,4'-(dithiodicarbonothioyl)dimorpholine 试剂级价格

CAS号:729-46-4
英文名:4,4'-(dithiodicarbonothioyl)dimorpholine
中文名:4,4'-(dithiodicarbonothioyl)dimorpholine
CBNumber:CB4875824
分子式:C10H16N2O2S4
分子量:324.51
MOL File:729-46-4.mol

4,4'-(dithiodicarbonothioyl)dimorpholine化学性质

熔点 :136-137 °C
沸点 :466.3±55.0 °C(Predicted)
密度 :1.4302 (rough estimate)
折射率 :1.5650 (estimate)
储存条件 :Store at -20°C
溶解度 :Soluble in DMSO
形态 :A solid
酸度系数(pKa) :0.58±0.20(Predicted)
颜色 :White to off-white
EPA化学物质信息 :Morpholine, 4,4'-(dithiodicarbonothioyl)bis- (729-46-4)

4,4'-(dithiodicarbonothioyl)dimorpholine性质、用途与生产工艺

生物活性 JX06 是一种细胞中的 PDK1 的选择性共价抑制剂。JX06 可剂量依赖性地抑制PDK1、PDK2和PDK3，对应的IC50值分别为0.049 μM、0.101 μM 和 0.313 μM。
靶点
Target Value
PDK1
(Cell-free assay) 0.049 μM
PDK2
(Cell-free assay) 0.101 μM
PDK3
(Cell-free assay) 0.313 μM

体外研究

JX06 barely shows inhibitory activity against PDK4 at a concentration of 10 μM.
JX06 (1-10 μM; 48 hours) induces cell apoptosis in cancer cells with high ECAR/OCR.
JX06 (0-0.6 μM; 72 hours) dose-dependently suppresses the growth of A549 cells.
JX06 (0.1-10 μM; 6-24 hours) inhibits PDHA1 phosphorylation in A549 cells in a time- and dose-dependent manner.
JX06 (1-10 μM) increases glucose uptake and intracellular ATP level and reduces aerobic glycolysis determined by the lactate production in A549 cells.
JX06 (1-10 μM; 24 hours) induces ROS generation in cancer cells with high extracellular acidification rate (ECAR)/ oxygen consumption rate (OCR) .

Apoptosis Analysis

Cell Line:	A549, EBC-1, HT-29 and H460 cells
Concentration:	0, 1, 3, 10 μM
Incubation Time:	48 hours
Result:	Induces cell apoptosis in A549 and EBC-1 cells.

Cell Viability Assay

Cell Line:	A549 cells
Concentration:	0, 0.2, 0.4, 0.6 μM
Incubation Time:	72 hours
Result:	Inhibits the viability of A549 cells in a dose dependent manner.

Western Blot Analysis

Cell Line:	A549 cells
Concentration:	0, 0.1, 0.3, 1, 3, 10 μM
Incubation Time:	0, 6, 12, 24 hours
Result:	Decreased PDHA1 phosphorylation at both serine 293 and serine 232 (S293 and S232) in a time- and dose-dependent manner.

体内研究

JX06 (40-80 mg/kg; i.p. for 21 days) inhibits tumor growth in vivo.

Animal Model:	A549 subcutaneous xenograft mice
Dosage:	40, 80 mg/kg
Administration:	I.p. injections for 21 days
Result:	Reduced tumor weights and 67.5% tumor volume at the dose of 80 mg/kg compared with the vehicle control. Well tolerated at the administration dose.