描述 宿主 种属反应性 验证的应用 品牌示例 背景
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ANTI-NEUN抗体

描述 宿主 种属反应性 验证的应用 品牌示例 背景
ANTI-NEUN抗体,,结构式
ANTI-NEUN抗体
  • CAS号:
  • 英文名:ANTI-NEUN
  • 中文名:ANTI-NEUN抗体
  • CBNumber:CB4484440
  • 分子式:
  • 分子量:0
  • MOL File:Mol file

ANTI-NEUN抗体性质、用途与生产工艺

  • 描述 Rabbit monoclonal to NeuN
  • 宿主 Rabbit
  • 种属反应性 与反应: Mouse, Rat, Human
  • 验证的应用 Suitable for: WB, IHC-P, IHC-Fr, IP
  • 品牌示例

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] (ab236870)

    Anti-NeuN antibody [EPR21906] (ab236870)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • 背景 NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
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