生物活性 靶点 体外研究 体内研究 EB-3D 试剂级价格
  • 首页
  • 个人中心
  • 委托采购
  • 采购清单
ChemicalBook  CAS数据库列表  EB-3D

EB-3D

生物活性 靶点 体外研究 体内研究 EB-3D 试剂级价格
EB-3D,1839150-63-8,结构式
EB-3D
  • CAS号:1839150-63-8
  • 英文名:EB-3D
  • 中文名:EB-3D
  • CBNumber:CB44667867
  • 分子式:C30H36BrN4O2+
  • 分子量:564.55
  • MOL File:1839150-63-8.mol
EB-3D化学性质
  • 储存条件 :Inert atmosphere,Room Temperature
  • 溶解度 :DMSO: 50 mg/mL (77.59 mM)
  • 形态 :Solid
  • 颜色 :White to off-white

EB-3D性质、用途与生产工艺

  • 生物活性 EB-3D 是一种有效的 choline kinase α (ChoKα) 的选择性抑制剂，对ChoKα1的IC50值为1 μM。EB-3D 可在白血病T细胞中诱导 AMPK-mTOR 通路失调和凋亡。
  • 靶点
    TargetValue
    ChoKα1
    (Cell-free assay)     		1 μM
  • 体外研究

    EB-3D displays (0-100 μM; 72 hours) excellent antiproliferative activity against a wide cohort of T-leukemic cell lines, with GI GI 50 s 13 values in the nanomolar range.
    EB-3D (1.25-5μM; 24 hours) induced apoptosis in leukemia cell lines.
    EB-3D (0.5-1 μM; 24 hours) induces a G0/G1 arrest that lead to apoptosis.
    EB-3D (0.3 μM; 48 hours) shows a first spike of activation of AMPKα after 30 minutes and a later increase in the phosphorylation of T172.
    EB-3D (1-40 μM; 48 hours) inhibits cell growth in HepG2 cells with a GI 50 of 14.55 μM.
    EB-3D induces deregulation of the AMPK-mTOR pathway and apoptosis in leukemia T-cells.

    Cell Proliferation Assay

    Cell Line: JURKAT, CCRF-CEM, HSB-2, MOLT-16, DNA-41, LOUCY, PEER, ALL-SIL cells
    Concentration: 0.001, 0.01, 0.1, 1, 10, 100 μM
    Incubation Time: 72 hours
    Result: Inhibited JURKAT, CCRF-CEM, HSB-2, MOLT-16, DNA-41, LOUCY, PEER, and ALL-SIL cells growth with GI 50 s of 136.2, 478.8, 17.7, 0.9, 60.6, 200, 265, and 132 nM, respectively.

    Apoptosis Analysis

    Cell Line: Jurkat, CCRF-CEM and HSB-2 cells
    Concentration: 1.25, 2.5, 5 μM
    Incubation Time: 24 hours
    Result: Induced apoptosis in leukemia cell lines.

    Cell Cycle Analysis

    Cell Line: Jurkat, CCRF-CEM and HSB-2 cells
    Concentration: 0.5, 1 μM
    Incubation Time: 24 hours
    Result: Induces cell cycle arrest in G0/G1 phase.

    Western Blot Analysis

    Cell Line: Jurkat cells
    Concentration: 0.3 μM
    Incubation Time: 48 hours
    Result: Showed a first spike of activation of AMPKα after 30 minutes of treatment and a later increase in the phosphorylation of T172. The increase in S79 phosphorylation of its main target ACC (acetyl-coenzyme A (CoA) carboxylase), followed the same pattern. This rapid activation of AMPK, in turn induced a consequent reduction in mTOR phosphorylation that is visible already at 30’ and that becomes amplified at longer time probably due to the interruption of feedback loops that are characteristic of mTOR connecting pathways.
  • 体内研究

    EB-3D (1 mg/kg; i.p.; every other day) impairs mammary tumor growth in syngeneic orthotopic E0771-C57BL/6 mouse model.
    EB-3D (2.5 mg/kg; every other day for 4 weeks) shows a reduction of the number of spontaneous lung macro- and micrometastasis.

    Animal Model: E0771-C57BL/6 mice
    Dosage: I.p.; every other day for 4 weeks
    Administration: 2.5 mg/kg
    Result: A reduction of the number of spontaneous lung macro- and micrometastasis.
EB-3D上下游产品信息
上游原料
下游产品
EB-3D 试剂级价格
  • 更新日期：2024/04/30
  • 产品编号：HY-115463
  • 产品名称：EB-3D
  • CAS编号：
  • 包装：1 mg
  • 价格：952元
  • 更新日期：2024/04/30
  • 产品编号：HY-115463
  • 产品名称：EB-3D EB-3D
  • CAS编号：1839150-63-8
  • 包装：5mg
  • 价格：1900元
EB-3D生产厂家
  • 公司名称：上海超岚化工科技中心
  • 联系电话：QQ:65489617 15618227136
  • 电子邮件：info@SuperLan-chem.com
  • 国家：中国
  • 产品数：9863
  • 优势度：58
  • 公司名称：HANGZHOU CLAP TECHNOLOGY CO.,LTD
  • 联系电话：86-571-88216897,88216896 13588875226
  • 电子邮件：sales@hzclap.com
  • 国家：中国
  • 产品数：6313
  • 优势度：58
关注Chemicalbook 进入官方账号