AUDA

生物活性靶点体外研究体内研究 AUDA 试剂级价格

AUDA

CAS号:479413-70-2
英文名:AUDA
中文名:AUDA
CBNumber:CB32590311
分子式:C23H40N2O3
分子量:392.58
MOL File:479413-70-2.mol

AUDA化学性质

熔点 :114 °C(Solv: hexane (110-54-3))
沸点 :592.7±19.0 °C(Predicted)
密度 :1.09±0.1 g/cm3(Predicted)
储存条件 :Sealed in dry,Store in freezer, under -20°C
溶解度 :DMSO: ≥10mg/mL at warmed to 60°C
形态 :powder
酸度系数(pKa) :4.78±0.10(Predicted)
颜色 :white to beige

安全信息

WGK Germany :3

AUDA性质、用途与生产工艺

生物活性 AUDA (compound 43) 是一种有效的 soluble epoxide hydrolase (sEH) 的抑制剂，对小鼠sEH和人sEH的IC50值分别为18 nM和69 nM。AUDA具有抗炎活性，可降低MMP-9、IL-1β、TNF-α 和 TGF-β。AUDA还可下调 Smad3 和 p38 信号通路。
靶点
Target Value
MMP-9
()
IL-1β
()
TNF-α
()
TGF-β
()
Smad3
()

体外研究

AUDA (0.3-10 μg/mL; 48 hours) dose-dependently suppresses the proliferation of rat VSMCs exposed to PDGF.
AUDA (0.3-10 μg/mL; 30 min) dose-dependently upregulats COX-2 expression.
AUDA (10, 50 and 100 μM) augments the migratory ability of HCAECs in a dose-dependent manner.
AUDA significantly increases the adhesion ability of HCAECs.

Cell Proliferation Assay

Cell Line:	Vascular smooth muscle cell (VSMC)
Concentration:	0.3, 1, 3, 10 μg/mL
Incubation Time:	48 hours
Result:	Dose-dependently suppressed the proliferation of rat VSMCs exposed to PDGF.

Western Blot Analysis

Cell Line:	VSMC
Concentration:	1, 3, 10, 30 μg/mL
Incubation Time:	30 min
Result:	Dose-dependently upregulated COX-2 expression.

体内研究

AUDA (i.p.; 10 mg/kg; 14 days) reduces TNF-α, MMP-9 and IL-1β expression levels.

Animal Model:	Male (wild-type) C57BL/6 mice (age, 4-6 weeks; weight, 18-20 g)
Dosage:	10 mg/kg
Administration:	i.p.; 14 days
Result:	Reduced TNF-α, MMP-9 and IL-1β expression levels.