Anti-JAK1抗体

Rabbit

与反应: Mouse, Rat, Human

Produced in rabbits immunized with E. coli-derived Human JAK1 fragment, and purified by antigen affinity chromatography.

Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

Lane 1: Wild-type HAP1 whole cell lysate (40 µg)

Lane 2: Empty

Lane 3: JAK1 (KO) whole cell lysate (40 µg)

Lane 4: A431 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green).

Green - ab133666 observed at 133 kDa. Red - loading control, ab8245, observed at 37 kDa. ab133666 was shown to specifically recognize JAK1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed

when JAK1 knockout samples were examined. Wild-type and JAK1 knockout samples were subjected to SDS-PAGE. Ab133666 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively.

Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

Synthetic peptide corresponding to residues in Human JAK1

Anti-JAK1抗体是一类可以特异性结合Anti-JAK1的多克隆抗体，主要用于检测Anti-JAK1的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。