CHAT胆碱乙酰转移酶抗体
- CAS号:
- 英文名:Anti-CHAT Polyclonal Antibody
- 中文名:CHAT胆碱乙酰转移酶抗体
- CBNumber:CB25530159
- 分子式:
- 分子量:0
- MOL File:Mol file
CHAT胆碱乙酰转移酶抗体性质、用途与生产工艺
- 产品描述 Anti-CHAT/Choline Acetyltransferase Antibody, Rabbit Polyclonal
- 宿主 Rabbit
- 种属反应性 Human
- 免疫原 A synthetic peptide corresponding to the center region of the Human Choline Acetyltransferase
- 经验证的应用 IHC-P,ICC/IF
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品牌示例
Anti-CHAT/Choline Acetyltransferase Antibody, Rabbit Polyclonal-Cat: 100420-T10 ---Sino Biological
Immunofluorescence staining of CHAT in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CHAT polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus. - 制备方法 Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Choline Acetyltransferase, and purified by antigen affinity chromatography.
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纯化方法
CHAT胆碱乙酰转移酶抗体的纯化方法:蛋白A亲和纯化。
- 背景 Congenital myasthenic syndromes (CMS) are neuromuscular transmission disorders caused by mutations in genes encoding neuromuscular junction proteins. CMS due to choline acetyltransferase (CHAT) gene is characterized by episodic apnea. The CHAT gene encodes choline acetyltransferase, which is an enzyme responsible for the biosynthesis of the neurotransmitter acetylcholine in the brain. The choline O-acetyltransferase (CHAT) gene has been associated with various human disorders that involve cognitive impairment or deficiency. Choline acetyltransferase (CHAT) gene mutations cause a rare presynaptic congenital myasthenic syndrome due to impaired acetylcholine resynthesis.
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应用
胆碱乙酰转移酶是一种在神经元胞体内合成的酶。当该转移酶被合成以后，通过轴质流动方式转移到神经轴突末端。其功能是将乙酰辅酶A转移到胆碱上，导致神经递质乙酰胆碱的形成。既往研究发现胆碱乙酰转移酶为MG胸腺的差异表达蛋白之一。CHAT作为非神经元型胆碱能系统的关键蛋白，近年来受到较多关注。
- 保存条件 CHAT胆碱乙酰转移酶抗体的保存条件:4℃运输，-20℃保存，避免反复冻融。
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简介
CHAT胆碱乙酰转移酶抗体是以CHAT胆碱乙酰转移酶为抗原的多克隆抗体，可以特异性结合CHAT胆碱乙酰转移酶。CHAT胆碱乙酰转移酶抗体主要用于ICC/IF,Dotblot,ELISA,IHC-P,IHC-Fr,Immunomicroscopy,WB等CHAT胆碱乙酰转移酶检测实验。
- 公司名称：北京索莱宝科技有限公司
- 联系电话：010-50973130 4009686088
- 电子邮件：3193328036@qq.com
- 国家：中国
- 产品数：29795
- 优势度：68
- 公司名称：北京博奥森生物技术有限公司
- 联系电话：400-901-9800 18611424007
- 电子邮件：cis@bioss.com.cn
- 国家：中国
- 产品数：9518
- 优势度：58
