ANTI-CD3抗体

兔单克隆抗体 to CD3

与反应: Mouse, Human

Synthetic peptide within Human CD3 aa 150 to the C-terminus. The exact sequence is proprietary.

适用于: Flow Cyt, IHC-P, WB, mIHC

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody(ab16669)

IHC image of CD3 staining in a formalin fixed, paraffin embedded normal rat spleen tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16669 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ANTI-CD3抗体是以CD3抗体为抗原的APC多克隆抗体，可以特异性结合CD3抗体。ANTI-CD3抗体主要用于ICC/IF,Dotblot,ELISA,IHC-P,IHC-Fr,Immunomicroscopy,WB等CD3抗体检测实验。