苦参新醇 A

生物活性靶点体外研究苦参新醇 A 试剂级价格

苦参新醇 A

CAS号:99217-63-7
英文名:Leachianone E
中文名:苦参新醇 A
CBNumber:CB22651419
分子式:C25H28O5
分子量:408.49
MOL File:99217-63-7.mol

苦参新醇 A化学性质

熔点 :175-178 °C
沸点 :581.7±50.0 °C(Predicted)
密度 :1.212±0.06 g/cm3(Predicted)
储存条件 :4°C, protect from light
溶解度 :Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
形态 :Cryst.
酸度系数(pKa) :7.66±0.40(Predicted)
颜色 :White to off-white

苦参新醇 A性质、用途与生产工艺

生物活性 Kushenol A (Leachianone E) 从苦参根中分离出来的。Kushenol A 是一种非竞争性酪氨酸酶 (tyrosinase) 抑制剂，可阻止 L-tyrosinase 转化为 L-DOPA，IC50 和 Ki 值分别为 1.1 μM 和 0.4 μM。Kushenol A 是一种类黄酮抗氧化剂，对 α-葡萄糖苷酶 (alpha-glucosidase) (IC50: 45 μM; Ki: 6.8 μM) 和 β-淀粉酶 (beta-amylase) 具有抑制作用。Kushenol A 被认为是化妆品针对皮肤美白和衰老的潜在酶抑制剂。
靶点

IC50: 1.1 μM (tyrosinase); 45 μM (alpha-glucosidase)

体外研究

Kushenol A (25 μM) exhibits a highly potent inhibitory activity on ABTS+ radical scavenging with an IC ₅₀ value of 9.7 ± 0.1 μM, and exhibits inhibits scavenging activity as a percentage 93.7% at 25 μM.Kushenol A (0-60 μg/ml; 24 hours) shows considerable cytotoxic effects against NSCLC cells, exhibits IC ₅₀ values of 5.3 μg/ml and 20.5 μg/ml for A549 and NCI‐H226 cells, respectively. It shows an IC ₅₀ value of 57.2 μg/ml for BEAS-2B cells.

Cell Viability Assay

Cell Line:	NSCLC cell lines, A549 and NCI-H226Normal human lung epithelial: BEAS-2B
Concentration:	0-60 μg/ml
Incubation Time:	24 hours
Result:	Exhibited considerable cytotoxic effects against NSCLC cells.