CHPG SODIUM SALT

生物活性靶点体外研究体内研究 CHPG SODIUM SALT 试剂级价格

CHPG SODIUM SALT

CAS号:1303993-73-8
英文名:(RS)-2-Chloro-5-hydroxyphenylglycinesodiumsalt
中文名:CHPG SODIUM SALT
CBNumber:CB22518684
分子式:C8H7ClNNaO3
分子量:225.6
MOL File:1303993-73-8.mol

CHPG SODIUM SALT化学性质

储存条件 :Store at RT
溶解度 :Soluble to 50 mM in water and to 50 mM in DMSO
形态 :Powder
颜色 :Brown

CHPG SODIUM SALT性质、用途与生产工艺

生物活性 CHPG sodium salt 是一个选择性的 mGluR5 激动剂，并且通过 TSG-6/NF-κB 途径减弱 BV2 小神经胶质细胞 SO2 诱导的氧化应激和炎症。 CHPG sodium salt 通过 ERK 和 Akt 途径在体外和体内预防创伤性脑损伤 (TBI)。
靶点

mGlu ₅

NF-κB

ERK

Akt

体外研究

CHPG sodium salt (10-500 µM; 24 hours) significantly increases the cell viability and decreases the LDH release after SO ₂ derivatives treatment.
CHPG sodium salt (0.5 mM; 30 mins ) protects BV2 cells against SO ₂ -induced apoptosis.
CHPG sodium salt (0.5 mM; 30 mins) treatment alone increases the expression of TSG-6 in both mRNA and protein levels.

Cell Viability Assay

Cell Line:	BV2 microglial cells
Concentration:	10, 50, 100 and 500 µM
Incubation Time:	24 hours
Result:	Increased the cell viability.

Apoptosis Analysis

Cell Line:	BV2 microglial cells
Concentration:	0.5 mM
Incubation Time:	30 mins
Result:	Protected BV2 cells against SO ₂ -induced apoptosis.

Western Blot Analysis

Cell Line:	BV2 microglial cells
Concentration:	0.5 mM
Incubation Time:	30 mins
Result:	Increased the expression of TSG-6 in both mRNA and protein levels.

体内研究

CHPG sodium salt (injection; 250 nM; for 7 days) reduces significantly cerebral lesion volume.

Animal Model:	Adult Sprague-Dawley male rats weighing 280-320 g
Dosage:	250 nM
Administration:	Injection; for 7 days
Result:	Reduced significantly cerebral lesion volume.