ANTI-RAT CD3, FITC

Anti-CD3抗体(FITC)

Rat

FITC

ANTI-RAT CD3, FITC recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.

适用于: Flow Cyt

与反应: Mouse, Horse, Chicken, Dog, Human, Pig, Monkey, Rhesus monkey

Synthetic peptide: ERPPVPNPDYEPC Sequence derived from cytoplasmic epitope of CD3.

ANTI-RAT CD3, FITC( eBioscience11-0030-85)

PloS one 2012 - Figure 3 Inflammatory cell infiltrations and proliferation of the epithelium in the ureters of F2 mice showing hydronephrosis. Diseased ureters of BDF2 mice showed severe mononuclear cell infiltrations predominated by B220-positive cells (panel b). B220-positive cells and CD3-positive cells were mainly observed in the perivascular portions at the adventitia of ureters (panels a and b). CD16-positive cells were localized from the muscular layer to the lamina propria (panel c). Gr-1-positive cells were diffusely observed in the ureter and the lumen of gland-like structures (panel d). Metachromatic mast cells were not detected in the mucosa but several were observed in adipose tissue beside the adventitia (panel e, arrows and inset). In the pelvic-ureteral junction (panel f), proliferative cell nuclear antigen (PCNA)-positive cells were observed in the basal layer of the ureter transitional epithelium (arrows) but PCNA-positive nuclei were not detected in those of the renal pelvis (arrowheads). The black line indicates the border between the ureter and the renal pelvis (RP). UL: ureteral lumen. CD3, B220, CD16, Gr-1, PCNA: immunohistochemistry. TB: toluidine blue stain.

The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

ANTI-RAT CD3,FITC是以RAT CD3为抗原的FITC多克隆抗体，可以特异性结合RAT CD3。ANTI-RAT CD3,FITC主要用于ICC/IF,Dotblot,ELISA,IHC-P,IHC-Fr,Immunomicroscopy,WB等RAT CD3检测实验。

This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.

CD3是一种重要的白细胞分化抗原，几乎所有的T细胞表面均存在。它由4个遗传因子和6个肽链组成，是构成T细胞抗原受体（TCR）的膜抗原。在抗原与TCR结合时，参与其信号向细胞内的传达，T细胞的分化，向辅助T细胞、杀伤T细胞的分化及活性化，分泌细胞因子、细胞凋亡的发现，配位子的组合等，是与发现各种各样的T细胞机能有关的重要的膜抗原。
D3是检出末梢血中成熟T细胞的最好的标记。因而，测定CD3阳性T细胞对评价免疫缺陷症（如T细胞缺乏症）、白血病、淋巴瘤的分型诊断非常有用。另一方面CD3单克隆抗体在脏器移植、骨髓移植时的免疫抑制疗法以及因重症自身免疫疾患时，以除去T细胞为目的的免疫调节疗法中被充分利用。

This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.