SULFOSUCCINIMIDYL OLEATE

生物活性体外研究体内研究 SULFOSUCCINIMIDYL OLEATE 试剂级价格

CAS号:135661-44-8
英文名:Sulfosuccinimidyl Oleate Sodium
中文名:SULFOSUCCINIMIDYL OLEATE
CBNumber:CB21564909
分子式:C22H37NO7S
分子量:459.6
MOL File:135661-44-8.mol

SULFOSUCCINIMIDYL OLEATE化学性质

熔点 :220-2220C
密度 :1.19±0.1 g/cm3(Predicted)
储存条件 :-20°C Freezer
溶解度 :DMSO (Slightly), Methanol (Slightly, Heated)
形态 :Solid
酸度系数(pKa) :-0.04±0.20(Predicted)
颜色 :White to Off-White
稳定性 :Hygroscopic

安全信息

海关编码 :2928009090

SULFOSUCCINIMIDYL OLEATE性质、用途与生产工艺

生物活性 Sulfosuccinimidyl oleate (Sulfo-N-succinimidyl oleate) 是一种长链脂肪酸，抑制脂肪酸向细胞转运。Sulfosuccinimidyl oleate 是一种有效且不可逆的线粒体呼吸链抑制剂。Sulfosuccinimidyl oleate 结合小胶质细胞表面上的 CD36 受体。具有抗炎作用。

体外研究

Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not alter the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly reduces the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability.
Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours).

Cell Viability Assay

Cell Line:	BV2 cells
Concentration:	20 μM and 50 μM
Incubation Time:	24 hours
Result:	Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5 ng/mL IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with Sulfosuccinimidyl oleate prevented it.

Western Blot Analysis

Cell Line:	BV2 cells
Concentration:	50 μM
Incubation Time:	24 hours
Result:	Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38.

体内研究

Sulfosuccinimidyl oleate (50 mg/kg; administered once by single oral gavage) significantly reduces the cortical ischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. In addition, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke.

Animal Model:	4-month-old male BALB/cABom mice with pMCAo model
Dosage:	50 mg/kg
Administration:	Administered once by single oral gavage
Result:	Reduced brain damage following ischemia. Attenuated infarct size.