描述 宿主 种属反应性 偶联物 制备 验证的应用 品牌示例 背景 检测原理
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ANTI-EGFR抗体

ANTI-EGFR抗体,,结构式
ANTI-EGFR抗体
  • CAS号:
  • 英文名:Anti-EGFR Antibody
  • 中文名:ANTI-EGFR抗体
  • CBNumber:CB15713564
  • 分子式:
  • 分子量:0
  • MOL File:Mol file

ANTI-EGFR抗体性质、用途与生产工艺

  • 描述 兔单克隆抗体 to EGFR
  • 宿主 Rabbit
  • 种属反应性 与反应: Mouse, Rat, Human
  • 偶联物 Unconjugated
  • 制备 Produced in rabbits immunized with purified, recombinant Mouse EGFR / HER1 / ErbB1 (rM EGFR / HER1 / ErbB1; Catalog#51091-M08H; Q01279; Met1-Ser647). EGFR / HER1 / ErbB1 specific IgG was purified by Mouse EGFR / HER1 / ErbB1 affinity chromatography.
  • 验证的应用 适用于: WB, IP, Flow Cyt, IHC-P, ICC/IF
  • 品牌示例

    Western blot - Anti-EGFR antibody [EP38Y] (ab52894)

    ANTI-EGFR抗体
    All lanes : Anti-EGFR antibody [EP38Y] (ab52894) at 1/10000 dilution (purified)
    Lane 1 : Rat liver lysates
    Lane 2 : Mouse lung lysates
    Lysates/proteins at 15 µg per lane.
    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution Predicted band size: 134 kDa Observed band size: 175 kDa

  • 背景 As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
  • 检测原理 双抗体夹心法测定标本中EGFR水平。用纯化的EGFR抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入EGFR,再与HRP标记的EGFR抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的EGFR呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中EGFR浓度。
    Anti-EGFR Antibody是一类可以特异性结合EGFR的多克隆抗体,主要用于检测EGFR的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
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ANTI-EGFR抗体生产厂家
  • 公司名称:默克生命科学
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  • 国家:中国
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