倒千里光碱

生物活性体外研究体内研究倒千里光碱试剂级价格

倒千里光碱

CAS号:480-54-6
英文名:RETRORSINE
中文名:倒千里光碱
CBNumber:CB1256406
分子式:C18H25NO6
分子量:351.39
MOL File:480-54-6.mol

倒千里光碱化学性质

熔点 :208-211 °C (lit.)
比旋光度 :D18 -17.6° (c = 1.99 in ethanol)
沸点 :485.2°C (rough estimate)
密度 :1.32±0.1 g/cm3 (20 ºC 760 Torr)
折射率 :1.5100 (estimate)
储存条件 :2-8°C
形态 :Solid
酸度系数(pKa) :12.00±0.40(Predicted)
颜色 :White to off-white
Merck :13,8253
InChIKey :BCJMNZRQJAVDLD-VMOBWIEOSA-N
CAS 数据库 :480-54-6
(IARC)致癌物分类 :3 (Vol. 10, Sup 7) 1987
EPA化学物质信息 :Retrorsine (480-54-6)

安全信息

危险品标志 :T
危险类别码 :25
安全说明 :22-36/37/39-45
危险品运输编号 :1544
WGK Germany :3
RTECS号 :VH7525000
危险等级 :6.1(a)
包装类别 :II
毒害物质数据 :480-54-6(Hazardous Substances Data)

倒千里光碱性质、用途与生产工艺

生物活性 Retrorsine 是一种天然存在的有毒吡咯利西啶生物碱。Retrorsine 可以与 DNA 结合，抑制肝细胞的增殖能力。Retrorsine 可用于肝细胞损伤的研究。

体外研究

Retrorsine (60-240 μM; 24 hours) significantly reduces HSEC-CYP3A4 cells viability and GSH levels, and increases formation of pyrrole-protein adducts.

Cell Viability Assay

Cell Line:	HSEC-CYP3A4 cells
Concentration:	60 μM, 120 μM , 240 μM
Incubation Time:	24 hours
Result:	Significantly decreased cell viability.

体内研究

Retrorsine (30 mg/kg; i.p.; twice) impairs liver regeneration in the PBL model not only by an S or G2/M phase block, but also by a block located before the G1/S transition of the cell cycle.

Animal Model:	Male Wistar rats (180±20 g), portal branch ligation (PBL) model
Dosage:	30 mg/kg
Administration:	Intraperitoneal injection, twice, separated by 2-week interval
Result:	Strongly impaired the liver weight gain, protein and DNA synthesis as well as induction of cell cycle related proteins in the regenerating lobes after PBL.