N,N-二亚硝基哌嗪
- CAS号:140-79-4
- 英文名:N,N'-DINITROSOPIPERAZINE
- 中文名:N,N-二亚硝基哌嗪
- CBNumber:CB0420996
- 分子式:C4H8N4O2
- 分子量:144.13
- MOL File:140-79-4.mol
- 熔点 :208-210°C
- 沸点 :262.72°C (rough estimate)
- 密度 :1.4165 (rough estimate)
- 折射率 :1.8500 (estimate)
- 储存条件 :-20°C Freezer, Under Inert Atmosphere
- 溶解度 :Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly)
- 形态 :Solid
- 酸度系数(pKa) :-4.82±0.70(Predicted)
- 颜色 :White to Pale Yellow
- 水溶解性 :5.765g/L(24 ºC)
- 稳定性 :Hygroscopic
- CAS 数据库 :140-79-4
- EPA化学物质信息 :Piperazine, 1,4-dinitroso- (140-79-4)
N,N-二亚硝基哌嗪性质、用途与生产工艺
- 生物活性 N,N'-Dinitrosopiperazine (1,4-Dinitrosopiperazine; DNP) 是一种致癌物 (carcinogen)，特异性针对鼻咽癌，可促进鼻咽癌的转移。N,N'-Dinitrosopiperazine 通过蛋白磷酸化调节多种信号通路，包括 LYRIC，在丝氨酸 568 位。
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体外研究
N,N'-Dinitrosopiperazine (0.5-100 μM; 48 hours) has no inhibitory effects on the labeled 6-10B cells, and LDH activity is not significantly altered by DNP treatment in the 0.5-8 μM concentration range. However, it is cytotoxic from the concentration 10 μM.N,N'-Dinitrosopiperazine (2-8 μM; 24 hours) induces 6-10B cell invasion and motility in a dose-dependent manner. At 6 μM, when compares to the control group, DNP increases cell invasion at 421.7% and cell motility is increased by 328.2%.N,N'-Dinitrosopiperazine (6 μM; 24 hours) increases the expression of phospho-LYRIC s568 and LYRIC expression in CNE1 cells.
Cell Viability Assay
Cell Line: The labeled 6-10B cells Concentration: 0.5, 1, 2, 4, 6, 8, 10, 20, 40, 80, or 100 μM Incubation Time: 48 hours Result: Had no inhibitory effects at the concentration 0-8 μM. Western Blot Analysis
Cell Line: The NPC cell line CNE1 Concentration: 6 μM Incubation Time: 24 hours Result: Increased phospho-LYRIC s568 and LYRIC expression. -
体内研究
N,N'-Dinitrosopiperazine (injected into the tail veins; 40 mg/kg; 30 days) inhibits cell motility and invasion, and facilitates NPC metastasis in vivo. From a IHC result, Phospho-LYRIC expression is higher in the metastatic tumors of DNP-treated mice than in those of the untreated control mice.
Animal Model: BABL/c nude mice injected with labeled 6-10B cell suspensions (1 × 10 4 cells) with or without DNP(40 mg/kg) Dosage: 40 mg/kg Administration: Injected into the tail veins; 30 days Result: Induced LYRIC phosphorylation at serine 568 associated with NPC metastasis in vivo.
- 更新日期：2024/11/08
- 产品编号：HY-136433
- 产品名称：1,4-二亞硝哌【口+井】 N,N'-Dinitrosopiperazine
- CAS编号：140-79-4
- 包装：5 mg
- 价格：600元
- 更新日期：2024/11/08
- 产品编号：HY-136433
- 产品名称：1,4-二亞硝哌【口+井】 N,N'-Dinitrosopiperazine
- CAS编号：140-79-4
- 包装：10 mg
- 价格：950元
- 公司名称：Carbosynth
- 联系电话：--
- 电子邮件：sales@carbosynth.com
- 国家：英国
- 产品数：6005
- 优势度：58
- 公司名称：Carbone Scientific CO., LTD
- 联系电话：--
- 电子邮件：sales@carbonesci.com
- 国家：英国
- 产品数：6666
- 优势度：30
