肝素锂

简介制备生物活性靶点体外研究肝素锂试剂级价格

肝素锂

CAS号:9045-22-1
英文名:HEPARIN LITHIUM SALT
中文名:肝素锂
CBNumber:CB0345862
分子式:n.a.
分子量:0
MOL File:Mol file

肝素锂化学性质

储存条件 :+15C to +30C
形态 :White solid
颜色 :White to off-white
水溶解性 :Water: 125 mg/mL
EPA化学物质信息 :Heparin, lithium salt (9045-22-1)

安全信息

危险品标志 :Xi
危险类别码 :36/37/38
安全说明 :22-24/25-36-26
WGK Germany :3
RTECS号 :MI0700000
F :3-10
海关编码 :29319090

肝素锂性质、用途与生产工艺

简介
肝素锂是一种化学物质，外观是白色至类白色粉末。肝素锂抗凝血浆与血清中TP、ASO、UA、ALT、Mg、Cl、TC、CRP 检测结果比较差异无统计学意义(P>0.05)。肝素锂抗凝血浆与血清中HBD、LDH、TBA 检测结果比较差异有统计学意义(P<0.05)。因此除HBD、LDH、TBA 外，肝素锂抗凝血浆与血清之间的相关性较好。因此，在生活检测中采用肝素锂抗凝血浆代替血清的可行性较高，可将其作为一种重要的检测方法。
制备
1）粗品肝素酶解：将粗品肝素钠溶解，调PH值为7.5‑9.0，升温至50‑55℃，加入肝素酶解胰酶，保温4‑5小时，调PH值 9.0‑11，升温至85℃～90℃后，冷却，过滤去除沉淀物，滤液用乙醇沉淀，收集沉淀物(A)；

2）去蛋白：步骤1所得沉淀物(A)用水溶解，搅拌，调 PH至9‑11，加入蛋白质絮凝沉淀剂，搅拌，静置，过滤去除沉淀，收集滤液(D)，滤液(D)调PH至11‑13值，搅拌，静置，过滤去除沉淀，收集滤液(E)，滤液(E)调PH值至中性，用乙醇沉淀，收集沉淀物(B)；

3）氧化脱色：将步骤2所得沉淀物(B)用水溶解，用过氧化氢氧化24‑48小时，调整PH至10.5‑11.5后，过滤去除沉淀物，收集滤液(F)，滤液(F)调PH值至6.5‑7.5，用乙醇沉淀，收集沉淀物(C)；

4）树脂交换：将步骤3所得沉淀物(C)用水溶解，加入 LiCl，搅拌溶解，通过树脂柱进行离子交换，收集过柱流出液，得肝素锂溶液；

5）沉淀、脱水、干燥：将步骤4所得肝素锂溶液，过滤，用乙醇沉淀，收集沉淀物(G)，沉淀物(G)脱水，然后真空干燥，粉碎，得肝素锂精品。
生物活性 Heparin Lithium salt 是一种抗凝剂，可与抗凝血酶III (ATIII) 可逆地结合。Heparin Lithium salt 显著抑制外泌体-细胞相互作用。
靶点

Antithrombin III
体外研究

Heparin is a potent anticoagulant drug based on its ability to accelerate the rate at which antithrombin inhibits serine proteases in the blood coagulation cascade. Heparin interactes most tightly with peptides containing a complementary binding site of high positive charge density. Heparin resembles DNA as both are highly charged linear polymers that behave as polyelectrolytes. Heparin is believed to function as an anticoagulant primarily through its interaction with AT III by enhancing AT-III-mediated inhibition of blood coagulation factors, including thrombin and factor Xa. Heparin binds to AT III and thrombin in a ternary complex, increasing the bimolecular rate constant for the inhibition of thrombin by a factor of 2000. Heparin is principally located in the granules of tissue mast cells that are closely associated with the immune response. Heparin makes numerous contacts with both FGF-2 and FGFR-1 stabilizing FGF–FGFR binding. Heparin also makes contacts with the FGFR-1 of the adjacent FGF–FGFR complex, thus seeming to promote FGFR dimerization.
用途常用的肝素抗凝剂，肝素的钠、钾、锂、铵盐，其中以肝素锂最好。