巴佛洛霉素C 1

生物活性体外研究体内研究巴佛洛霉素C 1 试剂级价格

巴佛洛霉素C 1

CAS号:88979-61-7
英文名:BAFILOMYCIN C1
中文名:巴佛洛霉素C 1
CBNumber:CB0320023
分子式:C39H60O12
分子量:720.89
MOL File:88979-61-7.mol

巴佛洛霉素C 1化学性质

储存条件 :Store at -20°C
溶解度 :DMSO: 5 mg/ml; Methanol: 5 mg/ml
形态 :White to light-brown solid.
颜色 :Yellow

巴佛洛霉素C 1性质、用途与生产工艺

生物活性 Bafilomycin C1 是从链霉菌中分离得到的大环内酯类抗生素 (antibiotic)。Bafilomycin C1 是一种有效的、特异性的、可逆的 vacuolar-type H+-ATPases 抑制剂。Bafilomycin C1 抑制革兰氏阳性菌 (bacteria) 和真菌 (fungi) 的生长。Bafilomycin C1 可诱导细胞凋亡 (apoptosis)，可用于肝细胞癌 (HCC) 的研究。

体外研究

Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner.Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells.Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle.

Cell Viability Assay

Cell Line:	SMMC7721 cell and HepG2 cell
Concentration:	0.33 μM, 1.1 μM, and 3.3 μM for SMMC77211.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time:	6 days
Result:	Retarded the cell growth.

Western Blot Analysis

Cell Line:	SMMC7721 cells
Concentration:	3.3 μM
Incubation Time:	24 hours
Result:	Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis

Cell Line:	SMMC7721 and HepG2 cells
Concentration:	3.3 μM; 10 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in SMMC7721 and HepG2 cells.

体内研究

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model.

Animal Model:	BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×10 ⁶ cells/100 μL)
Dosage:	0.2 mg/kg
Administration:	Subcutaneous injection; 20 days
Result:	Suppressed tumor growth of SMMC7721 tumor xenografts.