62687-22-3
基本信息
野菊花内酯对照品
野菊花内酯标准品
野菊花内酯,分析标准品
Yejuhualactone
HANDELIN
YEJUHUALACTONE
6,6'-dihydroxy-6,6',9,9'-tetramethyl-3'-methylene-2,2'-dioxo-2',3a,3',3a',4,5,5',6,6a,6',7,9a,9b,9',9a',9b'-hexadecahydro-4'H-spiro[azuleno[4,5-b]furan-3,10'-[6a,9]ethanoazuleno[4,5-b]furan]-5-yl acetate
Spiro[azuleno[4,5-b]furan-3(2H),10'-[4H-6a,9]ethanoazuleno[4,5-b]furan]-2,2'(3'H)-dione, 4-(acetyloxy)-3a,3'a,4,5,5',6,6',6a,7,9',9a,9'a,9b,9'b-tetradecahydro-6,6'-dihydroxy-6,6',9,9'-tetramethyl-3'-methylene-, (3R,3aR,3'aS,4S,6R,6'R,6aR,6'aR,9'R,9aR,9'aS,9bR,9'bS)-
物理化学性质
| 外观性状 | 白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于野菊花。 |
| 沸点 | 731.0±60.0 °C(Predicted) |
| 密度 | 1.34±0.1 g/cm3 (20 ºC 760 Torr) |
| 酸度系数(pKa) | 14.34±0.70(Predicted) |
| 形态 | Solid |
| 颜色 | White to off-white |
常见问题列表
Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling.
Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin.
RT-PCR
| Cell Line: | RAW 264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 5 hours |
| Result: | iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed. |
Western Blot Analysis
| Cell Line: | RAW 264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | |
| Result: | Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells. |
Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model .
| Animal Model: | Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan |
| Dosage: | 10 mg/kg, 20 mg/kg |
| Administration: | Oral administration; for 30 mintues |
| Result: | Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited. |