42424-50-0

基本信息
双链聚肌胞溶液
双链聚肌胞钠盐
聚肌苷酸-聚胞苷酸钠盐
聚肌苷 - 聚胞苷酸钠盐
卡那霉素-聚肌胞钠盐复合物
聚肌胞溶液(双链聚肌胞溶液)
POLYI:C双链聚肌胞钠盐
6.双链聚肌胞(POLYI:C .)
Poly(I:C) sodium
POLY I POLY C, NA
Polymyocyte sodium salt
POLY[I] POLY[C] SODIUM SALT
polyinosinic-polycytidylic acid sodium
polyI-polyC sodium salt compound solution
POLYINOSINIC-POLYCYTIDYLIC ACID SODIUM SALT
POLYINOSINIC ACID POLYCYTIDYLIC ACID, SODIUM SALT
POLYINOSINIC ACID + POLYCYTIDYLIC ACID, SODIUM SALT
物理化学性质
储存条件 | −20°C |
储存条件 | −20°C |
溶解度 | H2O:可溶,10mg/mL |
形态 | 冻干粉 |
颜色 | White to off-white |
生物来源 | synthetic (organic) |
水溶解性 | Water : 10 mg/mL (Need ultrasonic and warming) |
InChIKey | ACEVNMQDUCOKHT-WOQDZEJINA-N |
SMILES | O[C@@H]1[C@@H]([C@@H](COP(O)(O)=O)O[C@H]1N1C=CC(N)=NC1=O)O.O[C@@H]1[C@@H]([C@@H](COP(O)(O)=O)O[C@H]1N1C=NC2C(NC=NC1=2)=O)O |&1:1,2,3,11,22,23,24,32,r| |
应用领域
双链聚肌胞价格(试剂级)
报价日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
2025/02/08 | HY-135748 | 双链聚肌胞 Polyinosinic-polycytidylic acid sodium | 42424-50-0 | 1 mg | 248元 |
2025/02/08 | HY-135748 | 双链聚肌胞 Polyinosinic-polycytidylic acid sodium | 42424-50-0 | 5 mg | 560元 |
2025/02/08 | HY-135748 | 双链聚肌胞 Polyinosinic-polycytidylic acid sodium | 42424-50-0 | 10mg | 915元 |
常见问题列表
TLR3, RIG-I, MDA5, apoptosis
Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.
Cell Viability Assay
Cell Line: | WM793, WM278, WM239A, WM9 and 1205Lu cells |
Concentration: | 20 ng/mL |
Incubation Time: | 24 hours |
Result: | Strongly reduced viability from 100% in controls to 20%–50% within 24 hours. |
Apoptosis Analysis
Cell Line: | 1205Lu cells |
Concentration: | 200 ng/mL |
Incubation Time: | 24 hours |
Result: | Induced apoptosis in 1205Lu cells. |
RT-PCR
Cell Line: | 1205Lu cells |
Concentration: | 3 ng/mL |
Incubation Time: | 24 hours |
Result: | Induced IFN-β expression in melanoma cells. |
Western Blot Analysis
Cell Line: | 1205Lu cells |
Concentration: | 5 ng/mL |
Incubation Time: | 24 hours |
Result: | Revealed active subunits of caspase-9 and caspase-8 in melanoma cells. |
Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.