1644388-26-0
CAS
1644388-26-0
分子式
C24H27Cl2N7
分子量
484.42
MOL 文件
1644388-26-0.mol
结构式
常见问题列表
生物活性
EAD1 是一种有效的自噬 (autophagy) 抑制剂,在肺癌和胰腺癌细胞中具有抗增殖活性。EAD1 还诱导凋亡 (apoptosis)。
体外研究
EAD1 (0-200 μM; 72 hours) has antiproliferative activity against human cancer cell lines.
EAD1 (5-75 μM; 24 hours) induces apoptosis in the H460 cells in a concentration-dependent manner.
EAD1 (1-25 μM; 24 hour) increases the punctate LC3 signal in H460 cells.
Cell Proliferation Assay
Cell Line: | H460 and HCC827 nonsmall cell lung cancer (NSCLC) and BxPC3 pancreatic cancer cells |
Concentration: | 0, 50, 100, 150, 200 μM |
Incubation Time: | 72 hours |
Result: | IC 50 s of 11, 7.6, and 5.8 μM for H460, HCC827, and BxPC3 cells, respectively. |
Apoptosis Analysis
Cell Line: | H460 cells |
Concentration: | 5, 25, 50, and 75 μM |
Incubation Time: | 24 hours |
Result: | Induction of apoptosis. |
Western Blot Analysis
Cell Line: | H460 cells |
Concentration: | 1, 2.5, 5, 10, 25 μM |
Incubation Time: | 24 hours |
Result: | Caused concentration and time-dependent increases in LC3-II levels. |