916753-62-3

UBQ-2 NHS Ester is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBQ-2 NHS Ester has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Appearance: Dark purple solid
ex/em: 566 nm (absorbance peak)
Extinction coefficient (ε): 38000 L??mol??1??cm??1

BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Primary amine

580 nm

38,000 cm-1M-1

Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650